High Resolution Chromatography: A Practical Approach

Paperback | March 1, 1999

EditorPaul Millner

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The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimentalsituations to enable rapid and effective purification. The first four chapters deal with the instrumental aspects of high resolution chromatography starting with the initial clean up steps prior to separation in chapter 1. Chapter 2 deals with microscale techniques, then chapter 3 describes thedetector technologies that can determine information about the separated molecules. The final chapter in this section cover capillary electrophoresis and its associated techniques. The remaining chapters cover a range of chromatographic procedures based on the interaction of a specific ligand withits target protein or other macromolecule. Some chapters cover non-specific interactions using peptides, inhibitors, and antibodies as the affinity ligand while others focus on specific groups of molecules : oligosaccharides and glycosylated proteins, nucleotide-binding proteins, proteins bindingfree and chelated metal ions, and DNA binding proteins.

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From the Publisher

The molecular biology revolution has required the development of new chromatographic techniques and the optimization of original techniques to give reasonable quantities of protein at high resolutions. The aim of this volume is to provide the necessary information in most experimentalsituations to enable rapid and effective purificatio...

Paul Millner, School of Biochemistry and Molecular Biology, University of Leeds.

other books by Paul Millner

Format:PaperbackPublished:March 1, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636486

ISBN - 13:9780199636488

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Table of Contents

Separation by charge, size and hydrophobicityPractice and benefits of microcolumn HPLCDetection devicesCapillary electrophoresis of peptides and proteinsLectins as affinity probesNucleotide-and dye-ligand chromatographySynthetic peptides as affinity ligandsDrugs and inhibitors as affinity ligandsImmunoaffinity chromatographyStrategies and methods for purification of Ca2+ -binding proteinsPurification of DNA-binding proteins

Editorial Reviews

"Laboratory workers will find this a useful guide to the development of effective purification protocols" Aslib Book Guide"