HIV: A Practical Approach Volume 2: Biochemistry, Molecular Biology, and Drug Discovery: Hiv Volume…

Paperback | September 1, 1995

EditorJonathan Karn

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AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long wayfrom understanding and controlling AIDS. HIV 1 and HIV 2 are guides to studying HIV in the laboratory. HIV 1 describes studies of the virus and infected cells and covers techniques for handling HIV safely, for studying viral entry, replication, and neutralization, and basic immunologicaltechniques. HIV 2 explores the biochemistry and molecular biology of HIV. Methods for cloning and expressing most of the viral proteins are described, plus assays for studying enzyme function in vitro. It also includes a section on recent approaches to drug discovery.HIV research is highly multidisciplinary so a complete compendium of all related methods would have been unreadable. Instead, theses two volumes focus on the specialized techniques which are unique to studies of HIV. The books have been designed so that a novice entering HIV research can findclear instructions about how to perform standard experiments, such as assays for reverse transcriptase or antibodies to HIV. In addition the books include many novel methods which are described in detail for the first time.

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From the Publisher

AIDS is one of the most pressing medical emergencies of our era. Despite arguments to the contrary, the majority of AIDS researchers have agreed that there is a causal link between HIV infections and AIDS. However, it is now 12 years since the discovery of HIV and we are still a long wayfrom understanding and controlling AIDS. HIV 1...

J. Karn is at MRC Laboratory of Molecular Biology, Cambridge.
Format:PaperbackPublished:September 1, 1995Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:019963498X

ISBN - 13:9780199634989

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Table of Contents

Section 1: Biochemistry and molecular biology of viral proteins1. The HIV life cycle2. Reverse transcriptase3. Ribonuclease H4. Integrase5. Protease6. Expression of gag, envelope and other HIV antigens using Bacculovirus7. Envelope expression and purification8. HIV envelope expression and purification9. RNA binding assays for the regulatory proteins tat and rev10. Cellular and cell-free assays for tat11. Cellular assays for rev12. DNA binding proteins interacting with the viral LTR13. Nef14. Vpu15. VifSection 2: Drug discovery16. Cellular assays for antiviral drugs17. Inhibition of HIV by ribozymes and antisense oligonucleotides18. Gene therapy approaches to the inhibition of HIV

Editorial Reviews

`The two volumes are of exceptional value for laboratory workers studying the many different aspects of HIV and HIV disease. At an affordable price, these two volumes provide a wealth of information on standard techniques and on more sophisticated approaches for the study of the interactionbetween cellular and viral proteins. It is a comprehensive manual which serves as a solid basis for all investigators who are exploring this complex virus and the complex pathogenesis of HIV disease. Prferably in the form of multiple copies, it deserves a place on the bookshelf of any laboratoryworking with HIV.'M.A. Koch, Zentralblatt fur Bakteriologie 1996