HIV Protocols: Second Edition by Vinayaka R. PrasadHIV Protocols: Second Edition by Vinayaka R. Prasad

HIV Protocols: Second Edition

EditorVinayaka R. Prasad, Ganjam V. Kalpana

Paperback | November 19, 2010

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Despite major advances in HIV treatment, many areas require more study, in order to create efficacious, potent antiretrovirals that can suppress viral load completely and durably without toxic side effects, to define unknown drug targets and fine-tune known targets, and to better understand the interplay between viral and host factors. In "HIV Protocols, Second Edition", expert researchers provide clear, state-of-the-art methods for the study of HIV. Directed toward three specific goals, this text aims to document up-to-date protocols for select aspects of HIV biology, to bring together both virological and immunological approaches in a single, convenient volume, and to present a comprehensive account of a range of techniques not available in any existing HIV protocol book. As a volume in the highly successful Methods in Molecular BiologyT series, the chapters include brief introductions to the subject, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections containing priceless tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, "HIV Protocols, Second Edition" is an ideal guide to the wide array of techniques used in fundamental or applied research into the biology and pathogenesis of HIV-1.
Title:HIV Protocols: Second EditionFormat:PaperbackDimensions:472 pages, 10.24 × 7.6 × 0.01 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617378089

ISBN - 13:9781617378089

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Table of Contents

I. Preparation of Virus Particles and Their Analysis1. Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 GenomesKimdar Sherefa Kemal, Milan Reinis, Barbara Weiser, Harold Burger2. Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies David E. Ott3. Calculating HIV-1 Infectious Titer Using a Virtual TCID50 MethodYong Gao, Immaculate Nankya, Awet Abraha, Ryan M. Troyer, Kenneth N. Nelson, Andrea Rubio, and Eric J. Arts II. Methods to Study HIV-1 ReplicationSection 1. Early Events4. Cell-free Assays for HIV-1 UncoatingChristopher Aiken5. Real-Time PCR Analysis of HIV-1 Replication Post Entry EventsJean L. Mbisa, Krista A. Delviks-Frankenberry, James A. Thomas, Robert J. Gorelick and Vinay K. Pathak6. Analysis of 2-LTR Circle Junctions of Viral DNA in Infected CellsDibyakanti Mandal and Vinayaka R. Prasad7. HIV-1 Recombination: An Experimental Assay and a Phylogenetic ApproachMichael D. Moore, Mario P.S. Chin, and Wei-Shau Hu8. Methods of Preparation and Analysis of Intracellular Reverse Transcription ComplexesAriberto Fassati9. Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitationSergey N. Iordanskiy and Michael I. Bukrinsky10. Isolation and Analysis of HIV-1 Preintegration ComplexesAlan Engelman11. Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence MicroscopyNathalie J. Arhel and Pierre CharneauSection 2. Late Events12. Methods for the Study of HIV-1 AssemblyAbdul A. Waheed, Akira Ono and Eric O. Freed13. Assembly of Immature HIV-1 Capsids using a Cell-Free SystemJaisri R. Lingappa and Beth K. Thielen14. Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-like Particles in vitroSiddhartha A. K. Datta and Alan Rein15. Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with OligonucleotidesAndrew G. Stephen and Robert J. Fisher16. Methods for Analysis of Incorporation and Annealing of tRNALys in HIV-1 Shan Cen, Fei Guo, and Lawrence Kleiman III. Specialized Approaches to Study HIV-1 Biology and Pathogenesis17. Somatic Cell Genetic Analysis to Identify HIV-1 Host Restriction FactorsSusana T. Valente and Stephen P. Goff18. Rapid, Controlled and Intensive Lentiviral Vector-based RNAiManuel Llano, Natassia Gaznick and Eric M. Poeschla19. Reverse Two Hybrid Screening to Analyze Protein-protein Interaction of HIV-1 and Cellular ProteinsSupratik Das and Ganjam V. Kalpana20. Methods to Study Monocyte migration induced by HIV-infected cellsVasudev R. Rao, Eliseo Eugenin, Joan Berman and Vinayaka Prasad21. Novel Mouse Models for Understanding HIV-1 Pathogenesis Aviva Joseph, Kaori Sango, Harris Goldstein IV. Immunological Studies of HIVSection 1. Mucosal Immunology22. Mucosal Antibody Responses to HIVZina Moldoveanu and Jiri Mestecky23. Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology AssaysBarbara L. Shacklett, J. William Critchfield and Donna LemongelloSection 2. Measuring T cell Responses via Flow Cytometry24. Quantifying HIV-1-specific CD8+ T-cell Responses Using ELISPOT and Cytokine Flow CytometryBarbara L. Shacklett, J. William Critchfield and Donna Lemongello25. Multiparameter Flow Cytometry Monitoring of T cell ResponsesHolden T. MaeckerSection 3. Antiviral Responses26. Measuring HIV Neutralization in a Luciferase Reporter Gene AssayDavid C. Montefiori27. Assessing the Antiviral Activity of HIV-1-specific cytotoxic T lymphocytesOtto O. Yang28. Methods for Quantitating Antigen-specific T Cell Responses Using Functional Assays in Rhesus MacaquesRama Rao Amara V. Drug Resistant Viruses and Viral Evolution29. Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection Maureen Oliveira, Bluma G. Brenner, and Mark A. Wainberg30. Virus Evolution as a Tool to Study HIV-1 BiologyBen Berkhout and Atze T. Das