Human Monoclonal Antibodies: Methods and Protocols by Michael SteinitzHuman Monoclonal Antibodies: Methods and Protocols by Michael Steinitz

Human Monoclonal Antibodies: Methods and Protocols

EditorMichael Steinitz

Hardcover | September 14, 2013

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The introduction of monoclonal antibodies revolutionized immunology. The development of human monoclonal antibodies was inspired primarily by the enormous clinical benefits promised by these reagents which can be used as anti-inflammatory reagents, anti-tumor reagents and reagents for passive immunization in a variety of pathologies. Human Monoclonal Antibodies: Methods and Protocols presents technical protocols of cellular and molecular methods for the production, purification and application of human monoclonal antibodies, as well as review articles on related topics of human monoclonal and polyclonal antibodies. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Human Monoclonal Antibodies: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies which will prove invaluable in a clinical setting.
Title:Human Monoclonal Antibodies: Methods and ProtocolsFormat:HardcoverDimensions:390 pages, 10 × 7.01 × 0.01 inPublished:September 14, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035850

ISBN - 13:9781627035859

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Table of Contents

1. Human Monoclonal Antibodies - The Residual Challenge of Antibody ImmunogenicityHerman Waldmann 2. Technical and Ethical Limitations in Making Human Monoclonal AntibodiesMark C. Glassy and Rishab Gupta 3. Therapeutic Human Monoclonal Antibodies in Inflammatory DiseasesSotirios Kotsovilis and Evangelos Andreakos 4. Therapeutic Human Monoclonal Antibodies Against CancerJamie Jarboe, Anumeha Gupta, and Wasif Saif 5. Polyclonal and Monoclonal Antibodies in the ClinicBharath Wootla, Aleksandar Denic, and Moses Rodriguez 6. Production of Human Monoclonal Antibodies by the Epstein- Barr Virus MethodMichael Steinitz 7. Humanization and Simultaneous Optimization of Monoclonal AntibodyT. Kuramochi, T., Igawa, H. Tsunoda, and K. Hattori 8. Chimeric AntibodiesKohei Kurosawa, Waka Lin, and Kunihiro Ohta 9. Recombinant Genetic Libraries and Human Monoclonal AntibodiesJarrett J. Adams, Bryce Nelson, and Sachdev S. Sidhu 10. Production of Stabilized scFv Antibody Fragments in the E. coli Bacterial CytoplasmLilach Vaks and Itai Benhar 11. Construction and Production of an IgG-Like Tetravalent Bispecific Antibody, IgG-Single Chain Fv FusionDan Lu and Zhenping Zhu 12. Construction of Human Antibody Gene Libraries and Selection of Antibodies by Phage DisplayAndré Frenzel, Jonas Kügler, Sonja Wilke, Thomas Schirrmann, and Michael Hust 13. Antigen -Specific Human Monoclonal Antibodies from Transgenic MiceSusana Magadán Mompó and África González-Fernández 14. Phage Display Technology for Human Monoclonal AntibodiesCecilia Deantonio, Diego Cotella, Paolo Macor, Claudio Santoro, and Daniele Sblattero 15. Antigen-Specific in vitro Immunization: A Source for Human Monoclonal AntibodiesKosuke Tomimatsu and Sanetaka Shirahata  16. Methods for Radiolabelling of Monoclonal AntibodiesVladimir Tolmachev, Anna Orlova, and Karl Andersson 17. Purification of Human Monoclonal Antibodies and Their FragmentsThomas Muller-Späth and Massimo Morbidelli 18. Idiotype-Specific IVIG for Therapy of Autoimmune DiseasesTomer Bashi, Miri Blank, and Yehuda Shoenfeld