Human Papillomaviruses: Methods and Protocols by Clare DavyHuman Papillomaviruses: Methods and Protocols by Clare Davy

Human Papillomaviruses: Methods and Protocols

EditorClare Davy

Paperback | November 10, 2010

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Leading basic researchers and clinical scientists describe in detail a wide variety of established and cutting-edge techniques they have developed to study the lifecycle and biological properties of the human papillomavirus. The authors use these readily reproducible methods, ranging from PCR to propagation of HPV in vitro, to detect and type papillomavirus infections, study the papillomavirus lifecycle, and to produce and functionally analyze papillomavirus proteins. The protocols follow the successful Methods in Molecular MedicineT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Title:Human Papillomaviruses: Methods and ProtocolsFormat:PaperbackDimensions:512 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375268

ISBN - 13:9781617375262

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Table of Contents

Identification of New Papillomavirus TypesEthel-Michele de Villiers, Corinna Whitley, and Karin GunstIdentification of HPV VariantsJohn Cason, Jon Bible, and Christine MantHistochemical Analysis of Cutaneous HPV-Associated LesionsKiyofumi EgawaHistological Analysis of Cervical Intraepithelial NeoplasiaMichael Babawale, Rashmi Seth, Adam Christian, Wessam Al-Utayem, Ragini Narula, and David JenkinsDetection of Papillomavirus Proteins and DNA in Paraffin-Embedded Tissue SectionsWoei Ling Peh and John DoorbarDetection and Quantitation of HPV Gene Expression Using Real-Time PCRRashmi Seth, John Rippin, Li Guo, and David JenkinsAnalysis of p16INK4a and Integrated HPV Genomes as Progression MarkersSvetlana Vinokurova, Nicolas Wentzensen, and Magnus von Knebel DoeberitzUse of Biomarkers in the Evaluation of CIN Grade and Progression of Early CINJan P. A. Baak and Arnold-Jan KruseHPV DNA Detection and Typing in Cervical ScrapesPeter J. F. Snijders, Adriaan J. C. van den Brule, Marcel V. Jacobs, René P. Pol, and Chris J. L. M. MeijerHPV DNA Detection and Typing in Inapparent Cutaneous Infections and Premalignant LesionsMaurits de Koning, Linda Struijk, Mariet Feltkamp, and Jan ter ScheggetEstablishing HPV-Containing Keratinocyte Cell Lines From Tissue BiopsiesMargaret Anne StanleyUsing an Immortalized Cell Line to Study the HPV Life Cycle in Organotypic "Raft" CulturesPaul F. Lambert, Michelle A. Ozbun, Asha Collins, Sigrid Holmgren, Denis Lee, and Tomomi NakaharaDifferentiation of HPV-Containing Cells Using Organotypic "Raft" Culture or MethylcelluloseRegina Wilson and Laimonis A. LaiminsPropagation of Infectious, High-Risk HPV in Organotypic "Raft" CultureMargaret E. McLaughlin-Drubin and Craig MeyersRetrovirus-Mediated Gene Transfer to Analyze HPV Gene Regulation and Protein Functions in Organotypic "Raft" CulturesN. Sanjib Banerjee, Louise T. Chow, and Thomas R. BrokerThe HPV Xenograft Severe Combined Immunodeficiency Mouse ModelWilliam BonnezThe Cottontail Rabbit Papillomavirus Model of High-Risk HPV-Induced DiseaseJanet L. BrandsmaStudying the HPV Life Cycle in 3A Trophoblasts and Resulting PathophysiologyYong Liu, Hong You, and Paul L. HermonatReplication and Encapsidation of Papillomaviruses in Saccharomyces cerevisiaePeter C. AngelettiAnalysis of the Regulation of Viral TranscriptionBernd Gloss, Mina Kalantari, and Hans-Ulrich BernardAnalysis of HPV Transcription by RPAJason M. Bodily and Craig MeyersAnalysis of Regulatory Motifs Within HPV TranscriptsSarah A. Cumming and Sheila V. GrahamDetection of HPV Transcripts by Nested RT-PCRChristine Mant, Barbara Kell, and John CasonAnalysis of HPV DNA Replication Using Transient Transfection and Cell-Free AssaysBiing Yuan Lin, Thomas R. Broker, and Louise T. ChowDetection and Quantitation of HPV DNA Replication by Southern Blotting and Real-Time PCRIain M. Morgan and Ewan R. TaylorAnalysis of E7/Rb AssociationsSandra Caldeira, Wen Dong, and Massimo TommasinoTransformation Assays for HPV OncoproteinsPaola Massimi and Lawrence BanksAnalysis of Adeno-Associated Virus and HPV InteractionPaul L. Hermonat, Hong You, C. Maurizio Chiriva-Internati, and Yong LiuIn Vitro Assays of Substrate Degradation Induced by High-Risk HPV E6 OncoproteinsMiranda Thomas and Lawrence BanksMeasuring the Induction or Inhibition of Apoptosis by HPV ProteinsAnna M. Kowalczyk, Geraldine E. Roeder, Katie Green, David J. Stephens, and Kevin GastonCodon Optimization of Papillomavirus GenesMartin MüllerGeneration of HPV Pseudovirions Using Transfection and Their Use in Neutralization AssaysChristopher B. Buck, Diana V. Pastrana, Douglas R. Lowy, and John T. SchillerGeneration and Application of HPV Pseudovirions Using Vaccinia VirusMartin Sapp and Hans-Christoph SelinkaIndex