Hydrogen Peroxide and Cell Signaling, Part A

Other | June 19, 2013

byLester Packer, Lester Packer, Enrique Cadenas

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This new volume ofMethods in Enzymologycontinues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer.



  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers hydrogen peroxide and cell signaling
  • Contains chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer

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From the Publisher

This new volume ofMethods in Enzymologycontinues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and ...

ENRIQUE CADENAS, MD, PhD, received his PhD in biochemistry from the University of Buenos Aires, School of Medicine. He is professor of pharmacology and pharmaceutical sciences at the University of Southern California School of Pharmacy and of biochemistry and molecular biology at the University of Southern California Keck School of Med...

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Format:OtherDimensions:328 pages, 1 × 1 × 1 inPublished:June 19, 2013Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0124055427

ISBN - 13:9780124055421

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Table of Contents



      1. Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to Light
        Fiona A. Summers, Baozhong Zhao, Douglas Ganini, and Ronald P. Mason
      2. Boronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living Systems
        Vivian S. Lin, Bryan C. Dickinson and Christopher J. Chang
      3. Visualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent Probe
      4. In vivoImaging of H2O2Production inDrosophila
      5. Single Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell Imaging
      6. Electrochemical Biosensors for On-Chip Detection of Oxidative Stress
      7. Electrochemical Detection of H2O2 Formation in Isolated Mitochondria
      8. Detection of H2O2 by Fluorescence Correlation Spectroscopy
      9. Real-Time Monitoring of Reactive Oxygen and Nitrogen Species in a multi-well plate using the diagnostic marker products of specific probes
      10. H2O2 Delivery to Cells: Steady-State versus Bolus Addition
      11. Imaging H2O2 Microdomains in Receptor Tyrosine Kinases Signaling
      12. The Determination aAnd Analysis oOf Site-Specific Rates oOf Mitochondrial Reactive Oxygen Species Production
      13. A Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging
      14. Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.