Immune Homeostasis: Methods and Protocols by Andrew L. SnowImmune Homeostasis: Methods and Protocols by Andrew L. Snow

Immune Homeostasis: Methods and Protocols

EditorAndrew L. Snow, Michael J. Lenardo

Hardcover | February 9, 2013

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Many questions remain unresolved as researchers continue to characterize and define the nature of normal immune homeostasis and determine how these processes are dysregulated in immunodeficiency, as well as in autoimmune and lymphoproliferative disorders.  Immune Homeostasis: Methods and Protocols focuses on experimental techniques for measuring and analyzing immune cell dynamics, with a particular emphasis on examining lymphocyte programmed cell death in different contexts.  With contributions by leading experts in the field, the collection examines detailed protocols for studying various pathways of apoptosis and necrosis in different types of hematopoietic cells, both in vitro and in vivo, methods for studying the maintenance of lymphocyte populations in the steady-state or following infectious challenges in both mice and humans, and technical insights into state-of-the-art genomics tools, among other topics.  Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Valuable and easy to use, Immune Homeostasis: Methods and Protocols serves as an important toolkit for basic and clinical scientists interested in examining various aspects of immune homeostasis in both normal and disease-related contexts in order to further study the dynamic processes that contribute to homeostasis of the immune system.
Title:Immune Homeostasis: Methods and ProtocolsFormat:HardcoverDimensions:226 pagesPublished:February 9, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627032894

ISBN - 13:9781627032896

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Table of Contents

1. Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays            Lixin Zheng 2. Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells            Gil Katz and Andrew L. Snow 3. Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes            Joao Bosco Oliveira 4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique            Bernice Lo and Madhu Ramaswamy 5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation            Nelia Cordeiro and Nicolas Bidère 6. Analyses of Programmed Cell Death in Dendritic Cells            Min Chen, Lily Huang, and Jin Wang 7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity            Francis Ka-Ming Chan, Kenta Moriwaki, and María José De Rosa 8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers            Sema Kurtulus and David Hildeman 9. Homeostatic Proliferation of Mature T Cells            Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh 10. Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers            Liset Westera, Yan Zhang, Kiki Tesselaar, José A.M. Borghans, and Derek C. Macallan 11. Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis            Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, and Jacques J.M. van Dongen 12. Molecular Measurement of T Cell Receptor Excision Circles            Heather E. Lynch and Gregory D. Sempowski 13. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis            R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining 14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders            Yu Zhang and Helen C. Su 15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran 4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique            Bernice Lo and Madhu Ramaswamy 5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation            Nelia Cordeiro and Nicolas Bidère 6. Analyses of Programmed Cell Death in Dendritic Cells            Min Chen, Lily Huang, and Jin Wang 7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity            Francis Ka-Ming Chan, Kenta Moriwaki, and María José De Rosa 8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers            Sema Kurtulus and David Hildeman 9. Homeostatic Proliferation of Mature T Cells            Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh 10. Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers            Liset Westera, Yan Zhang, Kiki Tesselaar, José A.M. Borghans, and Derek C. Macallan 11. Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis            Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, and Jacques J.M. van Dongen 12. Molecular Measurement of T Cell Receptor Excision Circles            Heather E. Lynch and Gregory D. Sempowski 13. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis            R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining 14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders            Yu Zhang and Helen C. Su 15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran            Heather E. Lynch and Gregory D. Sempowski 13. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis            R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining 14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders            Yu Zhang and Helen C. Su 15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran            R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining 14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders            Yu Zhang and Helen C. Su 15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran 15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran            Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich 16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells            Dat Q. Tran            Dat Q. Tran