Immune Receptors: Methods and Protocols by Jonathan P. RastImmune Receptors: Methods and Protocols by Jonathan P. Rast

Immune Receptors: Methods and Protocols

EditorJonathan P. Rast, James W.D. Booth

Hardcover | June 29, 2011

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Immunology has made significant progress in the past decade, driven forward by rapidly advancing technology and a renewed interest in the vast realm of innate immunity. The receptors that mediate these functions are at the front lines of both protective and regulative roles of the immune system.  In Immune Receptors: Methods and Protocols, expert researchers present a variety of experimental approaches to the characterization of immune receptors and the cell biology that mediates their functions.  These include imaging techniques that aim to understand receptor localization and trafficking, techniques to measure receptor-ligand interactions, strategies to identify novel ligands and methods to analyze downstream receptor signaling, as well as strategies for genomic and proteomic characterization of receptor repertoires. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immune Receptors: Methods and Protocols offers technical descriptions and protocols that will be useful both to investigators who are interested in carrying out these procedures and to those who seek a deeper understanding of the bench science that lies behind the immunology literature.
Title:Immune Receptors: Methods and ProtocolsFormat:HardcoverDimensions:312 pagesPublished:June 29, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617791385

ISBN - 13:9781617791383

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Table of Contents

1. Screening for Ligands of C-Type Lectin-Like Receptors            Elwira Pyz and Gordon D. Brown 2. Yeast Surface Display of Lamprey Variable Lymphocyte Receptors (VLR)            Gang Xu, Satoshi Tasumi, and Zeev Pancer 3. Identification of Scavenger Receptor Ligands            Claudine Neyen, Annette Pluddemann, and Siamon Gordon 4. Construction, Expression, and Purification of Chimeric Protein Reagents Based on Immunoglobulin Fc Regions            John P. Cannon, Marci O'Driscoll, and Gary W. Litman 5. Innate Immune Receptors for Nucleic Acids            Andrea Stutz, Damien Bertheloot, and Eicke Latz 6. Analysis of Receptor-Ligand Interactions by Surface Plasmon Resonance            Kimiko Kuroki and Katsumi Maenaka 7. Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2            Birte Zurek, Harald Bielig, and Thomas A. Kufer 8. Determining Fc?RI Diffusional Dynamics Via Single Quantum Dot Tracking            Diane S. Lidke, Shalini T. Low-Nam, Patrick J. Cutler, and Keith A. Lidke 9. Ratiometric Analysis of Subcellular Recruitment of Fc Receptors During Phagocytosis            Patricia Mero and James W.D. Booth 10. Assessment of the Recycling of the Membrane-Bound Chemokine, CX3CL1            Sajedabanu Patel, Ilya Mukovozov, and Lisa A. Robinson 11. Measuring Immune Receptor Mobility by Fluorescence Recovery after Photobleaching            Kristen Silver and Rene E. Harrison 12. Probing the Plasma Membrane Structure of Immune Cells through the Analysis of Membrane Sheets by Electron Microscopy            Björn F. Lillemeier and Mark M. Davis 13. Rapamycin-Based Inducible Translocation Systems for Studying Phagocytosis            Michal Bohdanowicz and Gregory D. Fairn 14. Micropatterned Ligand Arrays to Study Spatial Regulation in Fc Receptor Signaling            Alexis J. Torres, David Holowka, and Barbara A. Baird 15. CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Antigens            Peter Chen, Aruz Mesci, and James R. Carlyle 16. Use of Colloidal Silica-Beads for the Isolation of Cell-Surface Proteins for Mass Spectrometry-Based Proteomics            Yunee Kim, Sarah Elschenbroich, Parveen Sharma, Lusia Sepiashvili, Anthony O. Gramolini, and Thomas Kislinger 17. Transfection-Based Genomic Readout for Identifying Rare Transcriptional Splice Variants            Larry J. Dishaw, M. Gail Mueller, Robert N. Haire, and Gary W. Litman 18. Characterizing Somatic Hypermutation and Gene Conversion in the Chicken DT40 Cell System            Nagarama Kothapalli and Sebastian D. Fugmann 19. Characterizing Immune Receptors from New Genome Sequences            Katherine M. Buckley and Jonathan P. Rast