Immunocytochemical Methods and Protocols by Constance OliverImmunocytochemical Methods and Protocols by Constance Oliver

Immunocytochemical Methods and Protocols

byConstance OliverEditorMaria Célia Jamur

Paperback | April 6, 2012

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Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing "Notes" provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.
Title:Immunocytochemical Methods and ProtocolsFormat:PaperbackDimensions:416 pagesPublished:April 6, 2012Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796824

ISBN - 13:9781617796821

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Table of Contents

Immunocytochemical Methods and Protocols, Third EditionConstance Oliver and Maria Célia Jamur, Eds. Table of ContentsPrefaceContributorsPart I. Antibody PreparationOverview of Antibodies for Immunochemistry Constance Oliver and Maria Célia JamurIntroduction to the Purification of Antibodies Ana Cristina Grodzki and Elsa BerensteinAntibody Purification: Ammonium Sulfate Fractionation or Gel FiltrationAna Cristina Grodzki and Elsa BerensteinAntibody Purification: Ion - Exchange chromatographyAna Cristina Grodzki and Elsa BerensteinAntibody Purification: Affinity Chromatography - Protein A and Protein G SepharoseAna Cristina Grodzki and Elsa BerensteinConjugation of Fluorochromes to AntibodiesSu-Yau MaoBiotinylation of AntibodiesSu-Yau MaoPart II. Tissue Preparation for Light Microscopic AnalysisCell Fixatives for ImmunostainingMaria Célia Jamur and Constance OliverPermeablization of Cell MembranesMaria Célia Jamur and Constance OliverPreparation of Frozen Sections for AnalysisGary L. BratthauerProcessing of Cytological SpecimensGary L. BratthauerProcessing of Tissue Culture CellsGary L. BratthauerProcessing Of Tissue SpecimensGary L. BratthauerHeat Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin SectionsLaszlo Krenacs, Tibor Krenacs, Eva Stekovics,and Mark RaffeldPart III: Bright Field Detection SystemsFluorochromes - Properties and Characteristics J. Michael MullinsDirect Immunofluorescent Labeling of CellsMaria Veronica Dávila PástorFluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture CellsMark C. WillinghamFluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture CellsMark C. WillinghamFluorescent Visualization of Macromolecules in Drosophila Whole MountsRicardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto ModaOverview of Conventional Fluorescence PhotomicrographyJ. Michael MullinsOverview of Confocal MicroscopyWilliam D. SwaimOverview of Laser Microbeam Applications as Related to Antibody Targeting P. Scott Pine Immuno-Laser Capture Microdissection of Rat Brain Neurons For Real Time Quantitative PCRL. Scot Bastian and Denis G. BaskinOverview of Antigen Detection through Enzymatic ActivityGary L. BratthauerThe Peroxidase-antiperoxidase (PAP) Method and Other All-Immunologic Detection MethodsGary L. BratthauerThe Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding MethodsGary L. BratthauerAvidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue Mark Raffeld and Elaine S. JaffeMultiple Antigen Immunostaining ProceduresTibor Krenacs, Laszlo Krenacs and Mark RaffeldImmunoenzymatic Quantitative Analysis of Antigens

Editorial Reviews

From the reviews of the third edition:"This book targets not only immunologists but a very broad readership. . very well-written and will be equally useful for a complete beginner to the technique, as well as for a scientist who has been using the methods for years. . I would recommend this book in particular to researchers who start to use immunocyto/histochemistry in their work . . The book should also be considered by laboratories that would like to have a reference for reliable immunostaining protocols." (Anna Vossenkaemper, Immunology News, August, 2010)