Immunocytochemical Methods and Protocols by Constance OliverImmunocytochemical Methods and Protocols by Constance Oliver

Immunocytochemical Methods and Protocols

EditorConstance Oliver, Maria Célia Jamur

Paperback | April 6, 2012

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The landscape of immunocytochemistry has significantly evolved in past years. Once a laborious technique with limited application, the introduction of new detection systems and instrumentation has facilitated the widespread use of immunocytochemical methods. In the post-genomic era, the use of immunocytochemical methods has grown to include the identification of a broad spectrum of antigens including proteins, carbohydrates and lipids from virtually every organism. In Immunocytochemical Methods and Protocols, Third Edition, expert researchers explore the modern methods employed in the field today,  now widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions, and to demonstrate the localization of subcellular components. Chapters provide methods that are designed to be general in their application and to appeal to investigators across all disciplines, including those in research and clinical settings. Composed in the highly successful Methods in Molecular BiologyT series format, chapters contain a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and ground-breaking,  Immunocytochemical Methods and Protocols, Third Edition, brings together a variety of methods that are normally limited to applications in a specific area, providing a unique and vital guide for researchers and clinicians alike.
Title:Immunocytochemical Methods and ProtocolsFormat:PaperbackDimensions:428 pagesPublished:April 6, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796824

ISBN - 13:9781617796821

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Table of Contents

Immunocytochemical Methods and Protocols, Third EditionConstance Oliver and Maria Célia Jamur, Eds. Table of ContentsPrefaceContributorsPart I. Antibody PreparationOverview of Antibodies for Immunochemistry Constance Oliver and Maria Célia JamurIntroduction to the Purification of Antibodies Ana Cristina Grodzki and Elsa BerensteinAntibody Purification: Ammonium Sulfate Fractionation or Gel FiltrationAna Cristina Grodzki and Elsa BerensteinAntibody Purification: Ion - Exchange chromatographyAna Cristina Grodzki and Elsa BerensteinAntibody Purification: Affinity Chromatography - Protein A and Protein G SepharoseAna Cristina Grodzki and Elsa BerensteinConjugation of Fluorochromes to AntibodiesSu-Yau MaoBiotinylation of AntibodiesSu-Yau MaoPart II. Tissue Preparation for Light Microscopic AnalysisCell Fixatives for ImmunostainingMaria Célia Jamur and Constance OliverPermeablization of Cell MembranesMaria Célia Jamur and Constance OliverPreparation of Frozen Sections for AnalysisGary L. BratthauerProcessing of Cytological SpecimensGary L. BratthauerProcessing of Tissue Culture CellsGary L. BratthauerProcessing Of Tissue SpecimensGary L. BratthauerHeat Induced Antigen Retrieval for Immunohistochemical Reactions in Routinely Processed Paraffin SectionsLaszlo Krenacs, Tibor Krenacs, Eva Stekovics, and Mark RaffeldPart III: Bright Field Detection SystemsFluorochromes - Properties and Characteristics J. Michael MullinsDirect Immunofluorescent Labeling of CellsMaria Veronica Dávila PástorFluorescence Labeling of Surface Antigens of Attached or Suspended Tissue-Culture CellsMark C. WillinghamFluorescence Labeling of Intracellular Antigens of Attached or Suspended Tissue-Culture CellsMark C. WillinghamFluorescent Visualization of Macromolecules in Drosophila Whole MountsRicardo Guelerman Pinheiro Ramos, Luciana Claudia Herculano Machado, and Livia Maria Rosatto ModaOverview of Conventional Fluorescence PhotomicrographyJ. Michael MullinsOverview of Confocal MicroscopyWilliam D. SwaimOverview of Laser Microbeam Applications as Related to Antibody Targeting P. Scott Pine Immuno-Laser Capture Microdissection of Rat Brain Neurons For Real Time Quantitative PCRL. Scot Bastian and Denis G. BaskinOverview of Antigen Detection through Enzymatic ActivityGary L. BratthauerThe Peroxidase-antiperoxidase (PAP) Method and Other All-Immunologic Detection MethodsGary L. BratthauerThe Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding MethodsGary L. BratthauerAvidin-Biotin Labeling of Cellular Antigens in Cryostat-Sectioned Tissue Mark Raffeld and Elaine S. JaffeMultiple Antigen Immunostaining ProceduresTibor Krenacs, Laszlo Krenacs and Mark RaffeldImmunoenzymatic Quantitative Analysis of Antigens Expressed on the Cell Surface (Cell-ELISA)Elaine Vicente Lourenço and Maria-Cristina Roque-BarreiraUse of Immunogold with Silver EnhancementConstance OliverPart IV: Fluorescence-Activated Cell Sorter (FACS) AnalysesOverview of Flow Cytometry and Fluorescent Probes for Flow CytometryRobert E. CunninghamTissue DisaggregationRobert E. CunninghamIndirect Immunofluorescent Labeling of Viable CellsRobert E. CunninghamIndirect Immunofluorescent Labeling of Fixed CellsRobert E. CunninghamFluorescent Labeling of DNARobert E. CunninghamDeparaffinization and Processing of Pathologic MaterialRobert E. Cunningham Part V: Colloidal Gold Detection Systems for Electron Microscopic AnalysisFixation and EmbeddingConstance Oliver and Maria Celia JamurPreparation of Colloidal GoldConstance OliverConjugation of Colloidal Gold to ProteinsConstance OliverColloidal Gold/Streptavidin MethodsConstance OliverPre-Embedding Labeling Methods Constance Oliver Postembedding Labeling Methods Constance Oliver  Part VI: The Clinical LaboratoryThe Clinical Immunohistochemistry Laboratory: Regulations and Troubleshooting Guidelines        Patricia A. Fetsch and Andrea Abati

Editorial Reviews

From the reviews of the third edition:"This book targets not only immunologists but a very broad readership. . very well-written and will be equally useful for a complete beginner to the technique, as well as for a scientist who has been using the methods for years. . I would recommend this book in particular to researchers who start to use immunocyto/histochemistry in their work . . The book should also be considered by laboratories that would like to have a reference for reliable immunostaining protocols." (Anna Vossenkaemper, Immunology News, August, 2010)