Immunodiagnostics: A Practical Approach

Paperback | November 25, 1999

EditorR. Edwards

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Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostictests are also used in other fields such as forensic science and environmental and food analysis. The different types of test range from simple manual methods to fully automated systems with sophisticated integrated detection. Immunodiagnostics: A Practical Approach starts off by explaining theprinciples and development of immunodiagnostic tests, specifically the use of radioisotopes as tracers. Chapter 2 explains the use of solid-phase supports to bind immunoreagents. Enzymes are widely used as labels in immunoassays and their use with colourimetric, fluorimetric, and chemiluminescentdetection systems is described. The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non- radioisotpoc prodcedures is the time-resolved fluorescence assay. Enzymes can also be used as a simple method of obtaining high performance from immunodiagnosticsand this application is covered later in the book. The next set of techniques to be described are light scattering techniques, which can be used in either simple manual assays or in sophisticated automated procedures. The penultimate chapter describes the principles of automation ofimmunodiagnostic tests. The last topic to be discussed is that of quality assurance.

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Immunodiagnostic tests are analytical methods that use antibodies as reagents whose results are used to aid diagnosis and are widely used in many scientific disciplines and in many different ways. Perhaps the most widespread and obvious use is in clinical applications, but immunodiagnostictests are also used in other fields such as for...

R. Edwards is at St Bartholomew's Hospital London.

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Format:PaperbackPublished:November 25, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199635889

ISBN - 13:9780199635887

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Table of Contents

1. Principles of Immunodiagnostic tests and their development (specifically the use of radioisotopes as tracers)2. Solid-Phase Supports3. Enzyme-labelled Tests with Colorimetric, Fluorimetric and Chemiluminescent Detection Systems4. Time-Resolved Fluorescence Immunosassay5. Light Scattering Techniques6. Enzyme Amplification7. Equipment and Automation; Appendix on Dose-Response Curve Fitting8. Quality Assurance in ImmunodiagnosticsIndex

Editorial Reviews

'....The book is certainly recommended to all immunologists working in basic immunology and in clinical laboratories.' Roald Nezlin