Immunological Methods

Kobo ebook | December 1, 2012

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Immunological Methods, Volume II compiles procedures that are appropriate for studies in immunology.

This book discusses the determination of equilibrium binding parameters of monoclonal antibodies specific for cell surface antigens; two-dimensional gel electrophoresis; and measurements of antibodies specific for DNA. The methods in surface physics for immunology; HLA-DR typing by complement-dependent B lymphocyte lysis; and protein A plaque assay for the detection of immunoglobulin-secreting cells are also elaborated.

This text also covers the in vitro production and testing of antigen-induced mediators of helper T-cell function; limiting dilution analysis of precursors of cytotoxic T lymphocytes; and induction of antibody formation in mouse bone marrow. Other topics include the long-term culture and cloning of specific helper T cells; cloning of alloreactive T cells; and enzyme immunoassay for the detection of hybridoma products.

This publication is valuable to immunologists and medical practitioners researching on immunological methods.

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Immunological Methods, Volume II compiles procedures that are appropriate for studies in immunology.This book discusses the determination of equilibrium binding parameters of monoclonal antibodies specific for cell surface antigens; two-dimensional gel electrophoresis; and measurements of antibodies specific for DNA. The methods in sur...

Format:Kobo ebookPublished:December 1, 2012Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:032314005X

ISBN - 13:9780323140058

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