Immunological Tolerance: Methods and Protocols by Paul J. FairchildImmunological Tolerance: Methods and Protocols by Paul J. Fairchild

Immunological Tolerance: Methods and Protocols

EditorPaul J. Fairchild

Paperback | November 19, 2010

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Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo. This guide is aimed at AIDS researchers, immunologists, transplant surgeons/specialists, and biotechnologists.
Title:Immunological Tolerance: Methods and ProtocolsFormat:PaperbackDimensions:492 pages, 9.02 × 5.98 × 0 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617376965

ISBN - 13:9781617376962

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Table of Contents

Immunological Tolerance: Methods and ProtocolsTable of ContentsIntroduction1. Frontiers of Immunological Tolerance Giorgio Raimondi, Heth Turnquist & Angus Thomson University of Pittsburgh Medical Center, Thomas E Starzl Transplantation Institute, Pittsburgh, USACell Types Contributing to Immunological Tolerance2. Case Study: Balancing Tolerance & Immunity: The Role of Dendritic Cell & T Cell SubsetsElena Chklovskaia & Barbara Fazekas de St GrothCentenary Institute of Cancer Medicine & Cell Biology, University of Sydney, Sydney, Australia3. Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow Ludovica BrunoThe Institute of Cancer Research, London, UK4. Genetic Modification of Dendritic Cells through the Directed Differentiation of Embryonic Stem Cells Paul J. Fairchild, Kathleen F. Nolan, &Herman WaldmannSir William Dunn School of Pathology, University of Oxford, Oxford, UK5. Generation of Immunocompetent T Cells from Embryonic Stem Cells Renée F. de Pooter & Juan-Carlos Zuñiga-PflückerDept of Immunology, University of Toronto, Sunnybrook & Women's Research Institute, Toronto, Canada6. Isolation, Expansion and Characterization of Human Natural and Adaptive Regulatory T Cells Silvia Gregori, Rosa Bacchetta,Laura Passerini, Megan K. Levings & Maria Grazia Roncarolo San Raffaele Telethon Institute for Gene Therapy, Milan, Italy7. Derivation, Culture and Characterization of Thymic Epithelial Cell Lines Michiyuki Kasai & Toshiaki MizuochiDept of Research on Blood & Biological Product, National Institute of Infectious Diseases, Tokyo, JapanThe Study of Immunological Tolerance in Vitro8. Case Study: Thymus Organogenesis and Development of the Thymic Stroma Craig Scott Nowell, Alison Mary Farley & Catherine Clare Blackburn Institute for Stem Cell Research, University of Edinburgh, Edinburgh, UK9. Case Study: Generation of a Tissue Engineered Thymic Organoid Fabrizio Vianello & Mark C. PoznanskyInfectious Diseases Division & Partners AIDS Research Center, Harvard Medical School, Charlestown, USA10. Studying T Cell Repertoire Selection Using Fetal Thymus Organ Cultures Philip G. Ashton-RickardtDepartment of Pathology & Ben May Institute for Cancer Research, University of Chicago, Chicago, USA11. Investigating Central Tolerance with Reaggregate Thymus Organ Cultures Graham Anderson & Eric J. JenkinsonMRC Centre for Immune Regulation, Department of Anatomy, University of Birmingham, Birmingham, UK12. Estimating Thymic Function through Quantification of T Cell Receptor Excision Circles Marie-Lise Dion, Rafick-Pierre Sékaly & Rémi CheynierLaboratoire d'Immunologie, Centre de Recherches du CHUM, Montréal, Canada13. Gene Expression Profiling of Dendritic Cells by Microarray Maria Foti, Paola Ricciardi-Castagnoli & Francesca GranucciDept of Biotechnology and Bioscience, University of Milano-Bicocca, Milan, Italy14. SAGE Analysis of Cell Types Involved in Tolerance Induction Kathleen F. Nolan, Stephen P. Cobbold & Herman WaldmannSir William Dunn School of Pathology, University of Oxford, Oxford, UK15. Analyzing the Physicodynamics of Immune Cells in a 3-D Collagen MatrixPeter Reichardt, Frank Gunzer & Matthias GunzerGerman Research Centre for Biotechnology, Braunschweig, GermanyThe Study of Immunological Tolerance in Vivo16. Case Study: Aetiology of Autoimmune Disease: How T Cells Escape Self-Tolerance Eli Sercarz & Claudia Raja-GabagliaTorey Pines Institute for Molecular Studies, San Diego, USA17. Case Study: Animal Models of Spontaneous Autoimmune Disease: Type 1 Diabetes in the Non-Obese Diabetic Mouse Nadia Giarratana, Giuseppe Penna & Luciano AdoriniBioXell, Milan, Italy18. Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis Mandy McGreachy, Richard O'Connor, Leigh A. Stephens & Stephen M. AndertonInstitute of Immunology and Infection Research, University of Edinburgh, Edinburgh, UK19. Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T Cell Subsets Kevin J. MaloySir William Dunn School of Pathology, University of Oxford, Oxford, UK20. In Vivo Models for the Study of Transplantation Tolerance Hannah Stewart, Rommel Ravanan & Richard Smith Academic Renal Unit, Southmead Hospital, Bristol, UK21. Ectopic Transplantation of Tissues under the Kidney Capsule Nathan Robertson, Paul J. Fairchild & Herman WaldmannSir William Dunn School of Pathology, University of Oxford, Oxford, UK22. Intravital Two Photon Imaging of T Cell Priming and Tolerance in the Lymph Node Susanna Celli & Philippe BoussoDépartment d'Immunologie, Institut Pasteur, Paris, France23. Tracing Tolerance and Immunity in Vivo by CFSE-Labeling of Administered Cells Elizabeth IngulliCenter for Immunology, University of Minnesota Medical School, Minneapolis, USAMethods for Inducing and Breaking Immunological Tolerance24. Case Study: Thymic Involution: Implications for Self-ToleranceFrances T. Hakim & Ronald E. GressExperimental Transplantation & Immunology Branch, National Cancer Institute, Bethesda, Maryland, USA25. Inducing Mixed Chimerism and Transplantation Tolerance through Allogeneic Bone Marrow Transplantation with Costimulation Blockade Ines Pree & Thomas WekerleDivision of Transplantation, Medical University of Vienna, Vienna, Austria26. Induction of Dominant Tolerance Using Monoclonal Antibodies Ana Água-Doce & Luis GraçaInstituto de Medicina Molecular, Universidade de Lisboa, Lisboa, Portugal27. Induction of Tolerance by Adoptive Transfer of Treg Cells Kanji Nagahama, Eiji Nishimura & Shimon Sakaguchi, Department of Experimental Pathology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan28. Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes Nicole R. Bianco, Seon-Hee Kim, Adrian E. Morelli & Paul D. RobbinsDept of Molecular Genetics and Biochemistry, University of Pittsburg School of Medicine, Pittsburg, USA29. Breaking Self-Tolerance to Tumor-Associated Antigens by in Vivo Manipulation of Dendritic Cells Ines Mende & Edgar G. EnglemanDepartment of Pathology, Stanford University School of Medicine, Palo Alto, USA * * *

Editorial Reviews

From the reviews:"Immunological Tolerance: Methods and Protocols unites the most recent ideas and techniques of outstanding scientists in this area. . I would recommended this book, not only as a way of getting into the field of immunological tolerance, but also as a laboratory resource for the techniques required. It provides a thorough guide to the field of immunological tolerance that will be helpful to both new and established scientists wishing to investigate this exciting field." (Carl Goodyear, Immunology News, February, 2008)"This guide provides clear information on modern laboratory techniques that are appropriate for the subject at hand -- immunological tolerance. In addition there are reviews of the literature on tolerance. . The audience for this book includes both basic science and clinical researchers who are studying problems relating to immunological tolerance. Experts in the field write the various chapters. . Thus, for anyone interested in studying tolerance, this is a potentially useful source of information." (Marion C. Cohen, Doody's Review Service, September, 2007)