Immunoproteomics: Methods and Protocols by Kelly M. FultonImmunoproteomics: Methods and Protocols by Kelly M. Fulton

Immunoproteomics: Methods and Protocols

EditorKelly M. Fulton, Susan M. Twine

Hardcover | August 21, 2013

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Providing current diverse approaches and techniques used to study the immunoproteome, Immunoproteomics: Methods and Protocols collects chapters from key researchers that deliver information to be used in diagnostics, disease progression, and vaccine correlates of protection analysis, to name but a few. This detailed volume includes techniques used for the study of the antibody targets of bacterial pathogens, viruses, and cancer, mass spectrometry-based approaches to characterize T-cell epitopes, chapters on detection and relative quantification of cytokines in serum, as well as in silico prediction of epitopes using sequence-based or modeling approaches.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Immunoproteomics: Methods and Protocols aids researchers in transferring these techniques to their own laboratories in addition to providing a reference to guide researchers toward appropriate techniques.
Title:Immunoproteomics: Methods and ProtocolsFormat:HardcoverDimensions:375 pages, 10 × 7.01 × 0.01 inPublished:August 21, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035885

ISBN - 13:9781627035880

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Table of Contents

1. Introduction to the Immune System            Scott McComb, Aude Thiriot, Lakshmi Krishnan, and Felicity Stark 2. Immunoproteomics: Current Technology and Applications            Kelly M. Fulton and Susan M. Twine 3. Antigen Identification Using SEREX            Ugur Sahin and Özlem Türeci 4. Antigen Discovery Using Whole-Genome Phage Display Libraries            Elisa Beghetto and Nicola Gargano 5. Methods and Applications of Serological Proteome Analysis            Kelly M. Fulton, Shannon S. Martin, Lawrence Wolfraim, and Susan M. Twine 6. Pre-Absorbed Immunoproteomics: A Novel Method for the Detection of Bacterial Surface Proteins            Guangjin Liu, Wei Zhang, and Chengping Lu 7. Identification of the Antigen Content of Electroimmunoprecipitates            N. Helena Beyer and Niels H.H. Heegaard 8. An Immunoproteomics Approach to Screen the Antigenicity of the Influenza Virus            Kevin M. Downard 9. In Vivo Microbial Antigen Discovery (InMAD) to Identify Diagnostic Proteins and Polysaccharides that Are Circulating During Microbial Infections            Sindy J. Chaves, Kathleen Schegg, Thomas R. Kozel, and David P. AuCoin 10. Chemo-Enzymatic Production of O-Glycopeptides for the Detection of Serum Glycopeptide Antibodies            Alexander Nøstdal and Hans H. Wandall 11. Enrichment and Characterization of Glycopeptide Epitopes from Complex Mixtures            Luc Tessier, Kelly M. Fulton, and Susan M. Twine 12. Whole-Cell MALDI-TOF Mass Spectrometry: A Tool for Immune Cell Analysis and Characterization            Richard Ouedraogo, Julien Textoris, Aurélie Daumas, Christian Capo, and Jean-Louis Mege 13. Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes            Richard H. Maier, Christina J. Maier, and Kamil Önder 14. Identification and Quantitation of MHC Class II-Bound Peptides from Mouse Spleen Dendritic Cells by Immunoprecipitation and Mass Spectrometry Analysis            Leonia Bozzacco and Haiqiang Yu 15. Construction and Screening of an Antigen-Derived Peptide Library Displayed on Yeast Cell Surface for CD4+ T Cell Epitope Identification            Fei Wen and Huimin Zhao 16. Profiling of Cytokine and Chemokine Responses Using Multiplex Bead Array Technology            Greg Harris and Wangxue Chen 17. Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis            Timothy J. Waybright, King C. Chan, Timothy D. Veenstra, and Zhen Xiao 18. Identification of Protein Biomarkers in Human Serum Using iTRAQ and Shotgun Mass Spectrometry            Theodoros A. Koutroukides, Julian A.J. Jaros, Bob Amess, Daniel Martins-de-Souza, Paul C. Guest, Hassan Rahmoune, Yishai Levin, Mike Deery, Philip D. Charles, Svenja Hester, Arnoud Groen, Andy Christoforou, Julie Howard, Nick Bond, Sabine Bahn, and Kathryn S. Lilley 19. Genome-Based Bioinformatic Prediction of Major Histocompatibility Complex (MHC) Epitopes            Simon J. Foote 20. Structure-Based Prediction of Major Histocompatibility Complex (MHC) Epitopes            Andrew J. Bordner 21. Prioritization of Therapeutic Targets of Inflammation Using Proteomics, Bioinformatics, and In Silico Cell-Cell Interactomics            Arsalan S. Haqqani and Danica B. Stanimirovic 22. Commercial Considerations for Immunoproteomics            Scott M. Ferguson