In Situ Detection of DNA Damage: Methods and Protocols by Vladimir V. DidenkoIn Situ Detection of DNA Damage: Methods and Protocols by Vladimir V. Didenko

In Situ Detection of DNA Damage: Methods and Protocols

EditorVladimir V. Didenko

Paperback | November 10, 2010

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In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. There are also novel instrumental techniques that use ultrasound for in vivo and in situ detection of apoptotic DNA damage, and in situ applications of flow and laser-scanning cytometry for analysis of DNA strand breaks and apoptosis. State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying the in situ detection of DNA damage and the labeling of apoptotic cells.
Title:In Situ Detection of DNA Damage: Methods and ProtocolsFormat:PaperbackDimensions:332 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372722

ISBN - 13:9781617372728

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Table of Contents

Part I. Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)Labeling DNA Damage with Terminal Transferase: Applicability, Specificity, and LimitationsP. Roy Walker, Christine Carson, Julie Leblanc, and Marianna SikorskaTUNEL Assay: An Overview of TechniquesDeryk T. LooElectron Microscopic Detection of DNA Damage Labeled by TUNELAntonio MigheliQuantitative Differentiation of Both Free 3' OH and 5' OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron MicroscopyYoshinori Otsuki and Yuko ItoDetermination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching TechniquesShinichi Ohno, Takeshi Baba, Nobuo Terada, and Yasuhisa FujiiIn Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning CytometryZbigniew Darzynkiewicz, Elzbieta Bedner, and Piotr SmolewskiPart II. Labeling DNA Breaks Using DNA Polymerase I or its Klenow FragmentDNA Damage Detection Using DNA Polymerase I or its Klenow Fragment: Applicability, Specificity, LimitationsJan Hein van DierendonckLabeling DNA Breaks In Situ by Klenow EnzymeKatherine A. WoodIn Situ Nick Translation at the Electron Microscopic LevelMarc ThiryPart III. Labeling DNA Breaks Using LigaseIn Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An OverviewPeter J. Hornsby and Vladimir V. DidenkoDetection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA LigaseVladimir V. DidenkoIn Situ Detection of Double-Strand DNA Breaks with Terminal 5'OH GroupsVladimir V. Didenko, Hop Ngo, and David S. BaskinPart IV. Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related TechniquesThe Comet Assay: Principles, Applications, and LimitationsAndrew R. CollinsThe Comet Assay: An Overview of TechniquesPeggy L. OliveUltrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL AssaysAndrei L. Kindzelskii and Howard R. PettyApplication of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH)José Luis Fernández and Jaime GosálvezPart V. Detection of Modified Bases and AP Sites in DNASimultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2'-Deoxyguanosine (8-OHdG)Alexander E. KalyuzhnyThe In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking TerminiPhilip K. Liu, Jiankun Cui, Niki Moore, and Dongya HuangPart VI. Indirect and General Markers of DNA DamageMarkers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA DamageYinong Zhou, Shi Liang, and Lawrence R. WilliamsUltrasound Imaging of Apoptosis: DNA-Damage Effects VisualizedGregory J. Czarnota, Michael C. Kolios, John W. Hunt, and Michael D. Sherarp53 Induction as an Indicator of DNA DamageGalina SelivanovaDetection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA)Zbigniew Darzynkiewicz, Elzbieta Bedner, Piotr Smolewski, Brian W. Lee, and Gary L. JohnsonIndex

Editorial Reviews

"Investigators working in the general area of DNA damage, including apoptosis, will find this book of use. The protocols are sufficiently detailed so as to allow experienced laboratory workers access to the described methods. . .the broad spectrum of methods described allows for individual investigators to exercise options."-Doody's Health Sciences Book Review Journal"Here, a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa)." - European Journal of Histochemistry