In Situ Hybridization: Principles and Practice

Hardcover | January 1, 1999

EditorJulia M. Polak, James OD. McGee

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This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take intoaccount the many advances in the last seven years.

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This practical guide covers all aspects of in situ hybridization, describing: practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. This new edition has been completely rewritten by the experts in the field to take intoaccount the many advances in the last seven year...

Julia Polak is at Royal Postgraduate Medical School, Hammersmith Hospital, London. James McGee is at University of Oxford Nuffield Department of Pathology and Bacteriology, John Radcliffe Hospital.

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Format:HardcoverDimensions:224 pages, 9.21 × 6.14 × 0.67 inPublished:January 1, 1999Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:019854880X

ISBN - 13:9780198548805

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Table of Contents

1. Principles of in situ hybridization2. The design and generation of probes for in situ hybridization3. The preparation of non-radioisotopic hybridization probes4. Principles and applications of complementary RNA probes5. Quantification of in situ hybridization6. Quantification of grains in in situ hybridization7. Detection of genetic changes in cancer by interphase cytogenetics and comparative genomic hybridization8. Single and simultaneous nucleic acid detection in clinical samples9. Combined in situ hybridization and immunocytochemistry: investigation of viral pathogenesis at the light and electron microscope levels10. Super sensitive in situ DNA and RNA localisation

Editorial Reviews

Anyone contemplating using in situ hybridisation in their laboratory will need a thorough grounding in the background and principles of the methods. This book excels in its background presentation, with clear explanations and good referencing.....There are very fewbooks dealing with thepracticalities of in situ hybridisation. This one makes a major contribution to the subject and therefore is invaluable as a reference for many scientists and clinicians Dr Deborah K Dun-Waters, The Bulletin of the Royal College of Pathologists, No. 107 June 1999