In Vitro Neurotoxicology: Methods and Protocols by Lucio G. CostaIn Vitro Neurotoxicology: Methods and Protocols by Lucio G. Costa

In Vitro Neurotoxicology: Methods and Protocols

EditorLucio G. Costa, Gennaro Giordano, Marina Guizzetti

Hardcover | July 25, 2011

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In recent years, the need to develop acceptable alternatives to conventional animal testing for neurotoxicity and developmental neurotoxicity has been increasingly recognized, and much effort is being directed toward the development of alternative models, utilizing mostly mammalian cells in culture but also non-mammalian model systems.  In Vitro Neurotoxicology: Methods and Protocols presents a series of cellular, biochemical, and molecular methodological protocols in the area of in vitro neurotoxicology, with an emphasis on mammalian cell culture systems.  Opening with a section on methodologies for preparing several cellular systems of variable complexity, amenable for in vitro neurotoxicological studies, the thorough volume continues with coverage of methods to measure cellular death and major mechanisms, methods for assessing mechanisms of nervous system cell toxicity related to impairment of cell signaling, while a final section illustrates additional methods for assessing important nervous system processes such as cell proliferation, neuritogenesis, and synaptogenesis.  Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, In Vitro Neurotoxicology: Methods and Protocols serves researchers with an interest in assessing or characterizing the potential neurotoxicity of environmental contaminants, drugs, or other chemicals.
Title:In Vitro Neurotoxicology: Methods and ProtocolsFormat:HardcoverDimensions:415 pagesPublished:July 25, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617791695

ISBN - 13:9781617791697

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Table of Contents

1. In Vitro Neurotoxicology: An Introduction            Lucio G. Costa, Gennaro Giordano, and Marina Guizzetti Part I: Model Systems 2. Primary Neurons in Culture and Neuronal Cell Lines for In Vitro Neurotoxicological Studies            Gennaro Giordano and Lucio G. Costa 3. Determination of Metal Interactions with the Chaperone Hspa5 in Human Astrocytoma Cells and Rat Astrocyte Primary Cultures            Evelyn Tiffany-Castiglioni, Yongchang Qian, Rola Barhoumi, and Ying Zheng 4. Microglia Cell Culture: A Primer for the Novice            Anke Witting and Thomas Möller 5. Neural Stem Cells for Developmental Neurotoxicity Studies            Roshan Tofighi, Michaela Moors, Raj Bose, Wan Norhamidah Wan Ibrahim, and Sandra Ceccatelli 6. Preparation, Maintenance, and Use of Serum-Free Aggregating Brain Cell Cultures            Paul Honegger, Antoinette Defaux, Florianne Monnet-Tschudi, and Marie-Gabrielle Zurich 7. Neurospheres as a Model for Developmental Neurotoxicity Testing            Ellen Fritsche, Kathrin Gassmann, and Timm Schreiber 8. Acute Hippocampal Slice Preparation and Hippocampal Slice Cultures            Pamela J. Lein, Christopher D. Barnhart, and Isaac N. Pessah 9. Glia-Neuron Sandwich Co-Cultures: An In Vitro Approach to Evaluate Cell-to-Cell Communication in Neuroinflammation and Neurotoxicity            Mariaserena Boraso and Barbara Viviani 10. In Vitro Models to Study the Blood Brain Barrier            Hilary Vernon, Katherine Clark, and Joseph P. Bressler Part II: Cell Death 11. Measurements of Cell Death in Neuronal and Glial Cells            Gennaro Giordano, Sungwoo Hong , Elaine M. Faustman, and Lucio G. Costa 12. Measurements of Neuronal Apoptosis            Gennaro Giordano and Lucio G. Costa 13. Measurement of Isoprostanes as Markers of Oxidative Stress            Dejan Milatovic, Thomas J. Montine, and Michael Aschner 14. Assessment of Glutathione Homeostasis            Gennaro Giordano, Collin C.  White, and Lucio G. Costa 15. Assessing Neuronal Bioenergetic Status            Stephanie L.H. Zeiger, Jeannette N. Stankowski, and BethAnn McLaughlin Part III: Signaling in Neurotoxicity 16. Fluorescent Assessment of Intracellular Calcium in Individual Cells            David P. Cox, Collin C. White, and Terrance J. Kavanagh 17. Homeostatic Regulation of Glutamate Neurotransmission in Primary Neuronal Cultures            Victor Briz and Cristina Suñol 18. Detection of Nitric Oxide Formation in Primary Neural Cells and Tissues            Ronald B. Tjalkens, David L. Carbone, and Guoyao Wu 19. JNK3-Mediated Apoptotic Cell Death in Primary Dopaminergic Neurons            Won-Seok Choi, Heather M Klintworth, and Zhengui Xia 20. Measurement of Proteasomal Dysfunction in Cell Models of Dopaminergic Degeneration            Faneng Sun, Anumantha G. Kanthasamy, and Arthi Kanthasamy 21. Cell Signaling and Neurotoxicity: Protein Kinase C In Vitro and In Vivo            Prasada Rao S. Kodavanti 22. Cell Signaling and Neurotoxicity: 3H-Arachidonic Acid Release (Phospholipase A2) in Cerebellar Granule Neurons            Prasada Rao S. Kodavanti Part IV: Other End-Points 23. Quantitative Assessment of Neurite Outgrowth in PC12 Cells            Joshua A. Harrill and William R. Mundy 24. Measurements of Astrocyte Proliferation            Marina Guizzetti, Terrance J. Kavanagh, and Lucio G. Costa 25. Quantification of Synaptic Structure Formation in Co-Cultures of Astrocytes and Hippocampal Neurons            Pamela J. Roqué, Marina Guizzetti, Gennaro Giordano, and Lucio G. Costa 26. Volume Measurements in Cultured Primary Astrocytes            Michael Aschner 27. Assessment of Cholesterol Homeostasis in Astrocytes and Neurons            Jing Chen, Lucio G. Costa, and Marina Guizzetti 28. Dose-Response or Dose-Effect Curves in In Vitro Experiments and Their Use to Study Combined Effects of Neurotoxicants            Matteo Goldoni and Sara Tagliaferri