In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols by Robert HoffmanIn Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols by Robert Hoffman

In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols

EditorRobert Hoffman

Hardcover | June 11, 2012

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The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology.  What was previously invisible in the cell often can be made visible with the use of fluorescent proteins. In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols presents state-of-the-art research that has contributed to the fluorescent protein revolution to visualize biological processes in the live animal. This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols is the first volume in the new field of in vivo cell biology and it serves both professionals and novices with its well-honed methodologies.
Title:In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and ProtocolsFormat:HardcoverDimensions:282 pagesPublished:June 11, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617797960

ISBN - 13:9781617797965

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Table of Contents

1. Assessing Cancer Cell Migration And Metastatic Growth In Vivo In The Chick Embryo Using Fluorescence Intravital Imaging                Hon Sing Leong, Ann F. Chambers, and John D. Lewis 2. The Use Of Fluorescent Proteins For Intravital Imaging Of Tumor Cell Invasion                James Hulit, Dmitriy Kedrin, Bojana Gligorijevic, David Entenberg,  Jeffrey Wyckoff, John Condeelis, and Jeffrey E. Segall 3. High Resolution In Vivo Imaging Of Fluorescent Proteins Using Window Chamber Models                Gregory M. Palmer, Andrew N. Fontanella, Siqing Shan, and Mark W. Dewhirst 4. In Vivo Imaging of Pancreatic Cancer with Fluorescent Proteins in Mouse Models                Michael Bouvet and Robert M. Hoffman 5. Lentivirus-Based DsRed-2-Transfected Pancreatic Cancer Cells for Deep In Vivo Imaging of Metastatic Disease                Zeqian Yu, Jiahua Zhou, and Robert M. Hoffman 6. Non-Invasive And Real-Time Fluorescence Imaging of Peritoneal Metastasis In Nude Mice                 Hayao Nakanishi, Seiji Ito, Makoto Matsui, Yuichi Ito, Kazunari Misawa, Yasuhiro Kodera  7. Three-Dimensional  In Vivo Imaging Of Tumors Expressing  Red Fluorescent Proteins                Alexander P. Savitsky, Irina G. Meerovich, Viktoria V. Zherdeva, Lyaisan R. Arslanbaeva, Olga S. Burova, D.V. Sokolova. E.M. Treshchalina, Anatoly Y. Baryshnikov, Ilya I. Fiks, Anna G. Orlova, Mikhail S. Kleshnin, Ilya V. Turchin, and Alexander M. Sergeev 8. Real Time Visualization and Characterization of Tumor Angiogenesis and Vascular Response to Anti-Cancer Therapies                H. Rosie Xing and Qingbei Zhang 9. In Vivo Imaging of Human Cancer with Telomerase-Specific Replication-Selective Adenovirus                Toshiyoshi Fujiwara 10. Real-Time Fluorescent Imaging of Abdominal, Pleural, and Lymphatic Metastases                Susanne Carpenter and Yuman Fong 11. Real-Time Imaging Of Tumors Using Replication-Competent Light-Emitting Microorganisms                Yong A. Yu, Stephanie Weibel, Aladar A. Szalay 12. GFP-Transgenic Animals For In Vivo Imaging: Rats, Rabbits And Pigs                 Takashi Murakami and Eiji Kobayashi 13. The Use Of Fluorescent Proteins For Developing Cancer-Specific Target Imaging Probes                Thomas McCann, Nobuyuki Kosaka, Peter L. Choyke, and Hisataka Kobayashi 14. In Vivo Imaging Of The Developing Mouse Embryonic Vasculature                Irina V. Larina and Mary E. Dickinson 15. Screening Reef Corals For Novel GFP-Type Fluorescent Proteins By Confocal Imaging                Anya Salih 16. What Does It Take To Improve Existing Fluorescent Proteins For In Vivo Imaging Applications?                Marc Zimmer 17. In Vivo Imaging of Oligonucleotide Delivery                Fumitaka Takeshita, Ryou-u Takahashi, Jun Onodera, and Takahiro Ochiya 18. Subcellular Imaging In Vivo:  The Next GFP Revolution                Robert M. Hoffman