Inflammation Protocols by Paul G. WinyardInflammation Protocols by Paul G. Winyard

Inflammation Protocols

EditorPaul G. Winyard, Derek A. Willoughby

Paperback | November 10, 2010

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Highly skilled experimenters present key techniques for the multidisciplinary study of inflammation in such conditions as inflammatory bowel disease, rheumatoid arthritis, renal disease, and cardiovascular disease. Described in step-by-step detail to ensure experimental success, the techniques are focused on the research and development of potential new antiinflammatory drugs in active target areas. The protocols are suitable for many current areas of drug discovery research, including transcription factors, cytokines, adhesion molecules, cyclooxygenase-2 (COX-2) inhibitors, free radicals, nitric oxide synthases, angiogenesis, wound healing, complement activation, immune rejection, and metalloproteinases.
Title:Inflammation ProtocolsFormat:PaperbackDimensions:390 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737282X

ISBN - 13:9781617372827

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Table of Contents

Part I. In Vitro Systems for Studying Aspects of the Inflammatory Response and Testing Antiinflammatory DrugsKey Stages in the Acute Inflammatory Response and Their Relevance as Therapeutic Targets: Introduction to Part 1Paul G. WinyardIkB Kinase and NF-kB Signaling in Response to Pro-Inflammatory CytokinesMireille DelhaseScreening for Inhibitors of Transcription Factors, Using Luciferase Reporter Gene Expression in Transfected CellsDeborah Phippard and Anthony M. ManningAdhesion Molecule Expression on Cytokine-Stimulated Human Endothelial CellsSusan L. Cuvelier and Kamala D. PatelPhagocytosis by Inflammatory Phagocytes: Experimental Strategies for Stimulation and QuantificationM. Rachel Morris, Sharon Dewitt, Iraj Laffafian, and Maurice B. HallettCytosolic Ca2+ Measurement and Imaging in Inflammatory CellsSharon Dewitt, Iraj Laffafian, M. Rachel Morris, and Maurice B. HallettDetection and Visualization of Oxidase Activity in PhagocytesMaurice B. Hallett, Caroline Cole, and Sharon DewittMeasurement of Complement ActivationTom Eirik MollnesMeasurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using ZymographyLinda Troeberg and Hideaki NagaseMeasurement of Aggrecanase-Generated Interglobular Domain Catabolites in the Medium and Extracts of Cartilage Explants Using Western Blot AnalysisClare E. Hughes, Christopher B. Little, and Bruce CatersonIn Vitro Model of Human Articular Cartilage DegradationWilliam D. ShingletonPart II. In Vivo Models of InflammationIn Vivo Models of Inflammation: Introduction to Part 2Derek A. WilloughbyCarrageenan-Induced Paw Edema in the Rat and MouseChristopher J. MorrisPleural Models of Inflammation: Immune and NonimmuneAdrian R. MooreModels of Acute Inflammation in the EarMiklós GáborMigration of Specific Leukocyte Subsets in Response to Cytokine or Chemokine Application In VivoMauro Perretti and Stephen J. GettingInflammatory Joint Disease: Clinical, Histological, and Molecular Parameters of Acute and Chronic Inflammation and Tissue DestructionNancy L. McCartney-Francis, James Chan, and Sharon M. WahlThe Assessment of Inflammation, Cartilage Matrix, and Bone Loss in Experimental Monoarticular Arthritis of the RatMichael P. SeedCollagen-Induced ArthritisAdrian R. MooreAir-Pouch Models of Inflammation and Modifications for the Study of Granuloma-Mediated Cartilage DegradationPaul Colville-Nash and Toby LawrenceQuantitative Analysis of Angiogenesis Using the Murine Chronic Granulomatous Air PouchChandan A. S. AlamModels of Coronary Artery Occlusion and Reperfusion for the Discovery of Novel Antiischemic and Antiinflammatory Drugs for the HeartNicole S. Wayman, Michelle C. McDonald, Prabal K. Chatterjee, and Christoph ThiemermannAssessment of Anticolitic Drugs in the Trinitrobenzene Sulfonic Acid (TNBS) Rat Model of Inflammatory Bowel DiseaseBrendan J. R. Whittle, Maryan Cavicchi, and Dominique LamarqueAn In Vivo Model of Ischemia/Reperfusion and Inflammation of the Kidneys of the RatPrabal K. Chatterjee and Christoph ThiemermannIn Vivo Models of Inflammation: Immune Rejection and Skin Transplantation In VivoIsabelle Binet and Kathryn J. WoodWound Healing: A Model of Dermal Wound RepairAnnette Tomlinson and Mark W. J. FergusonPart III. Pharmacodynamic Endpoints in Experimental Models and in Clinical Studies in HumansAn Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans: Introduction to Part 3David R. Blake and Gordon J. TaylorA Reply to "An Iconoclastic Approach to Pharmacodynamics in Model Systems: Their Relevance to Humans"Derek A. WilloughbyQuantifying Inflammation In Vivo Using Radiolabeled Antibodies and LeukocytesDiane Marshall and Dorian O. HaskardImmunoperoxidase Histochemistry for the Detection of Cellular Adhesion Molecule, Cytokine, and Chemokine Expression in the Arthritic SynoviumZoltan Szekanecz and Alisa E. KochRoles of Nitric Oxide and Superoxide in InflammationDaniela Salvemini, Harry Ischiropoulos, and Salvatore CuzzocreaAnalysis of Nitrite and Nitrate in the Study of InflammationClaire A. Davies, Sophie A. Rocks, Meg C. O'Shaughnessy, David Perrett, and Paul G. WinyardIn Vivo Assays for COX-2Chi-Chung ChanMeasurement of 8-epi-PGF2a as a Marker of Lipid Peroxidation In Vivo by Immunoaffinity Extraction and Gas Chromatography-Mass SpectrometryNitin K. Gopaul and Erik E. ÄnggårdLaboratory Assessment of the Acute Phase Response: Using CRP as a Model, Robert F. Ritchie and Thomas B. LedueAssays of Matrix Metalloproteinases (MMPs) and MMP Inhibitors: Bioassays and Immunoassays Applicable to Cell Culture Medium, Serum, and Synovial FluidJon B. Catterall and Tim E. CawstonIndex