Influenza Virus: Methods and Protocols by Yoshihiro KawaokaInfluenza Virus: Methods and Protocols by Yoshihiro Kawaoka

Influenza Virus: Methods and Protocols

byYoshihiro KawaokaEditorGabriele Neumann

Hardcover | April 18, 2012

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Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals - ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.

Title:Influenza Virus: Methods and ProtocolsFormat:HardcoverDimensions:234 pagesPublished:April 18, 2012Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796204

ISBN - 13:9781617796203

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Table of Contents

1. Influenza Viruses - An Introduction

                Yoshihiro Kawaoka and Gabriele Neumann


2. Influenza Virus Isolation

                Scott Krauss, David Walker, and Robert G. Webster


3. Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection

                Alexander Klimov,  Amanda Balish, Vic Veguilla, Hong Sun, Jarad Schiffer, Xiuhua Lu, Jacqueline M. Katz, and Kathy Hancock


4. Diagnosis of Influenza Virus

                Kirsten St. George


5. Solid-phase Assays of Receptor-binding Specificity

                Mikhail N. Matrosovich and Alexandra S. Gambaryan


6. The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors

                Margaret Okomo-Adhiambo, Aeron C. Hurt, and Larisa V. Gubareva


7. The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors

                Aeron C. Hurt, Margaret Okomo-Adhiambo, and Larisa V. Gubareva


8. Animal Models

                Edwin J.B. Veldhuis Kroeze, Thijs Kuiken, and Albert D.M.E. Osterhaus


9. Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture

                Klaus Stöhr, Doris Bucher, Tony Colgate, and John Wood


10. Genetic Engineering of Live Attenuated Influenza Viruses

                Hong Jin, Zhongying Chen, Jonathan Liu, and George Kemble


11. Influenza A Virus Molecular Virology Techniques

                Bin Zhou and David E. Wentworth


12. Reverse Genetics of Influenza Viruses

                Gabriele Neumann, Makoto Ozawa, and Yoshihiro Kawaoka


13. Genetic Analysis

                Gavin J.D. Smith, Justin Bahl, and Vijaykrishna Dhanasekaran

Editorial Reviews

From the reviews:"The chapters are all extremely informative both for a new researcher in the field or for the more seasoned flu researcher, and I think this book would be a valuable addition to the laboratory bookshelf. . This really is a multi-disciplinary book, which I personally think is very beneficial, as influenza researchers need to be able to approach research from all angles to try to stay one step ahead of an ever evolving virus." (Ruth Harvey, Microbiology Today, November, 2012)"This book describes methods to diagnose and culture influenza viruses as well as procedures used to evaluate antiviral drugs and to evaluate and produce vaccines to new viral strains. It provides detailed, step-by-step procedures for all aspects of influenza research. . Researchers in the field of virology are the intended audience. The techniques described in the book have been used in numerous laboratories around the world and will be useful for students as well as established scientists." (Rebecca T. Horvat, Doody's Book Reviews, July, 2012)