Influenza Virus: Methods and Protocols by Yoshihiro KawaokaInfluenza Virus: Methods and Protocols by Yoshihiro Kawaoka

Influenza Virus: Methods and Protocols

EditorYoshihiro Kawaoka, Gabriele Neumann

Hardcover | April 18, 2012

Pricing and Purchase Info

$180.95 online 
$193.95 list price save 6%
Earn 905 plum® points

Prices and offers may vary in store


In stock online

Ships free on orders over $25

Not available in stores


Reports of influenza-like illnesses date back to the Middle Ages, and outbreaks of influenza likely afflicted humans long before that. Over the last half century, influenza virus research has led to the development of two classes of antivirals - ion channel and neuraminidase inhibitors. Recently, a method of the artificial generation of an influenza virus was established. This system has been instrumental in the development of novel influenza vaccines and in the understanding of viral pathogenicity and the functions of viral proteins. Influenza Virus: Methods and Protocols summarizes the current techniques that have made this progress possible, ranging from protocols for virus isolation, growth, and subtyping to procedures for the efficient generation of any influenza virus. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Influenza Virus: Methods and Protocols seeks to serve both professionals and novices with the techniques used in numerous laboratories around the world that are, thus, the building blocks that underpin almost all influenza virus research.
Title:Influenza Virus: Methods and ProtocolsFormat:HardcoverDimensions:244 pagesPublished:April 18, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796204

ISBN - 13:9781617796203

Look for similar items by category:


Table of Contents

1. Influenza Viruses - An Introduction                Yoshihiro Kawaoka and Gabriele Neumann 2. Influenza Virus Isolation                Scott Krauss, David Walker, and Robert G. Webster 3. Influenza Virus Titration, Antigenic Characterization, and Serological Methods for Antibody Detection                 Alexander Klimov,  Amanda Balish, Vic Veguilla, Hong Sun, Jarad Schiffer, Xiuhua Lu, Jacqueline M. Katz, and Kathy Hancock 4. Diagnosis of Influenza Virus                Kirsten St. George 5. Solid-phase Assays of Receptor-binding Specificity                 Mikhail N. Matrosovich and Alexandra S. Gambaryan 6. The Chemiluminescent Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors                Margaret Okomo-Adhiambo, Aeron C. Hurt, and Larisa V. Gubareva 7. The Fluorescence Neuraminidase Inhibition Assay: A Functional Method for Detection of Influenza Virus Resistance to the Neuraminidase Inhibitors                Aeron C. Hurt, Margaret Okomo-Adhiambo, and Larisa V. Gubareva 8. Animal Models                Edwin J.B. Veldhuis Kroeze, Thijs Kuiken, and Albert D.M.E. Osterhaus 9. Influenza Virus Surveillance, Vaccine Strain Selection and Manufacture                Klaus Stöhr, Doris Bucher, Tony Colgate, and John Wood 10. Genetic Engineering of Live Attenuated Influenza Viruses                 Hong Jin, Zhongying Chen, Jonathan Liu, and George Kemble 11. Influenza A Virus Molecular Virology Techniques                Bin Zhou and David E. Wentworth 12. Reverse Genetics of Influenza Viruses                Gabriele Neumann, Makoto Ozawa, and Yoshihiro Kawaoka 13. Genetic Analysis                Gavin J.D. Smith, Justin Bahl, and Vijaykrishna Dhanasekaran

Editorial Reviews

From the reviews:"The chapters are all extremely informative both for a new researcher in the field or for the more seasoned flu researcher, and I think this book would be a valuable addition to the laboratory bookshelf. . This really is a multi-disciplinary book, which I personally think is very beneficial, as influenza researchers need to be able to approach research from all angles to try to stay one step ahead of an ever evolving virus." (Ruth Harvey, Microbiology Today, November, 2012)