Interleukin Protocols by Luke A. J. O'NeillInterleukin Protocols by Luke A. J. O'Neill

Interleukin Protocols

EditorLuke A. J. O'Neill, Andrew Bowie

Paperback | November 9, 2010

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Luke O'Neill and Andrew Bowie bring together a collection of standard and advanced methods for measuring these powerful agents. Their readily reproducible techniques range from the assay of interleukin protein and mRNA-using ELISA, FACS, and RT-PCR-to the study of interleukin signal transduction. Newer techniques are also covered, including the analysis of interleukin gene polymorphisms and the use of cDNA microarrays. Many of the assays are geared to specific pathologies, including breast cancer, depression, psoriasis, Grave's disease, migraine, and myocardial infarction. Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics.
Title:Interleukin ProtocolsFormat:PaperbackDimensions:460 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371629

ISBN - 13:9781617371622

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Table of Contents

Part I: General Methods for Assaying Interleukin Protein and mRNAELISAs and Interleukin ResearchCatherine GreeneELISPOT Technique for Assaying InterleukinsAlmuth Christine Hauer and Mona Bajaj-ElliottMeasurement of Interleukins by ELISPOT Assay with Particular Application for Dual Color Analysis (Stardust Assay)Yoshihiro Okamato and Mikio NishidaAssaying Interleukins Intracellularly by FACSLiam O'Mahony and Maurice O'DonoghueBasic RT-PCR for Measurement of Cytokine ExpressionMona Bajaj-Elliot and Almuth Christine HauerQuantitative PCR for Measurement of Cytokine ExpressionEllen M. Nilsen, Finn-Eirik Johnasen, and Per BrandtzaegSingle Cell RT-PCR Method for the Detection of Cytokine mRNAMoshe Ligumsky, Hovav Nechushtan, and Ehud Razin.uantitation of Cytokine mRNA by Flash-Type BioluminescenceJeffrey K. ActorMeasurement of Interleukin mRNA by Northern Blotting Using a Nonradioactive Detection MethodGinette Tardif, John A. Di Battista, Jean-Pierre Pelletier, and Johanne Martel-PelletierQuantification of Mouse IL-6 and TNF-_ mRNA Using Xplore AssaysScott W. Van Arsdell, Kevin P. Murphy, Csaba Pazmany, Diane Erickson and Mark D. MoodyMeasurement of Cytokine and Chemokine mRNA Using Nonisotopic MultiProbe RNase Protection AssayZheng-Ming Wang and Roman DziarskiIsolation of Total and Bioactive Interleukins by Immunoaffinity-Receptor Affinity ChromatographyTerry M. Phillips and Benjamin F. DickensDetection of Cytokines by ImmunohistochemistryMahmoud HuleihelWhole Blood Assays and the Influence of Circadian Rhythmicity on Human Cytokine MeasurementNicolai PetrovskyPart II: Illustration of Methodologies Using Individual InterleukinsAssaying Interleukin-8Paul N. MoynaghMeasurement of Interleukin-2 by BioassayLyanne WestonAssaying Interleukin-15Amos Douvdevani, Michal Weiler, and Eli LewisPart III: Assaying Interleukins in Particular PathologiesAssaying Interleukin-6 in Breast CancerJudith Harmey, Amanda Haverty, Deirdre Foley and David Bouchier-HayesCytokines, Stress, and Depressive IllnessHymie Anisman, Shawn Hayley, Arun V. Ravindran, Zul Merali, and Jenna GriffithsMeasurement of Interleukins in Cutaneous DisordersKhusru Asadullah, Antje Haeussler-Quade, Wolf Dietrich Doecke, Wolfram SterryInterleukins in Graves' DiseaseJan KomorowskiEvaluation of Cytokines in Migraine Patients by ELISAIrene Munno, Mariarosaria Marinaro, Giuseppe Lacedra, Antonia Bassi, and Vincenzo CentonzeAssaying Interleukins in Plasma in the Course of Acute Myocardial InfarctionRadek PudilPart IV: Assaying Interleukins in Particular Biological FluidsMeasurement of Cytokines in Peritoneal FluidsReijo Punnonen and Juha PunnonenMeasurement of Cytokines in Seminal PlasmaMarek Glezerman, Eitan Lunenfeld, and Mahmoud HuleihelMeasurement of Cytokines in Induced Sputum: Application to the Investigation of Asthma and COPDPeter G. Gibson and Jodie L. SimpsonCytokines in Blister FluidFranco Ameglio and Luciano D'AuriaMeasurement of Cytokines in Synovial FluidFabrizio De BenedettiMeasurement of Cytokines in Cerebrospinal FluidToshiyuki YokoyamaDetection of Interleukins in TearsArchana Thakur and Mark D. P. WillcoxPart V: Newer Techniques: Interleukin Signal Transduction, Recombinant Interleukin Production, Interleukin Gene Polymorphisms, and the use of cDNA ArraysMeasurement of Phosphoinositide 3-Kinase Activation by InterleukinsStephen G. WardAnalysis of Interleukin-2 Signaling Using Affinity Precipitations and Polyacrylamide Gel ElectrophoresisPaul Brennan and Veronica Athie-MoralesApplications of Green Fluorescent Protein to Cell SignalingPaul Brennan and Emmanuelle AstoulUse of Pichia pastoris for the Production of Recombinant CytokinesKevin P. Murphy, Csaba Pazmany, and Mark D. MoodyAnalysis of Single Nucleotide Polymorphisms in the Interleukin-1B Gene Using 5' Nuclease AssaysEmad M. El-OmarMeasurement of Cytokine and Chemokine Gene Expression Patterns Using cDNA ArrayZheng Ming-Wang and Roman Dziarski

Editorial Reviews

"this volume offers protocols for measurement of various interleukins. Most are of sufficient detail so as to serve as a laboratory guide for investigators. Investigators with a specific interest in analyzing a given interleukin will find this volume helpful. "-Doody's Health Sciences Book Review Journal"Comprehensive and highly practical, Interleukin Protocols offers biomedical investigators a stellar collection of all the major techniques needed to analyze the role of interleukins in disease, to improve diagnosis, and to foster the rapid emergence of new and more powerful therapeutics." - Journal of Investigational Allergology and Clinical immunology"A variety of techniques is represented in 36 chapters which are written in a concise, uniform format b y authors who obviously have a lot of hands-on experience with the methods covered...If you are working in this area, put this book on a shelf in the lab and it will not be just recently started graduate students who will gracefully turn to it. - Chembiochem"The protocols that are described in the book are user friendly, in the sense that they are not only well detailed and easy to follow but also reliable. The researchers that are naive in the field will find a lot of useful information in the book whereas the experienced ones will be able to use the book as a source of additional information on the methods already in use. A must therefore for the psychoneuroendocrinologist with a working interest in the relationships between the (neuroendocrine) brain and the immune system." -Psychoneuroendocriniology