Introduction to Flow Cytometry: INTRO TO FLOW CYTOMETRY by James V. WatsonIntroduction to Flow Cytometry: INTRO TO FLOW CYTOMETRY by James V. Watson

Introduction to Flow Cytometry: INTRO TO FLOW CYTOMETRY

byJames V. Watson

Paperback | November 11, 2004

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Flow cytometry is a technique for measuring both scattered light and fluorescence from single cells at very rapid rates. Typically up to 5000 cells can be analysed per second. Using various fluorochromes this allows a cell population to be analysed for cells showing certain characteristics such as the presence of a particular enzyme, cellular constituent or other gene product. The information it can provide is invaluable in helping to diagnose certain cancers as well as aiding pure research into many aspects of cell biology such as the cell cycle and gene expression. This book describes the technology in a simple and direct way. The fundamental concepts upon which the technology is based are outlined and the book goes on to describe flow cytometers and what can be gained from using them in biology and medicine. The book gives a particularly detailed account of how artifactual results can arise and where 'noise' is generated. Anyone starting to use, or already using this technique, will need to read this book.
Title:Introduction to Flow Cytometry: INTRO TO FLOW CYTOMETRYFormat:PaperbackDimensions:460 pages, 9.72 × 6.85 × 0.94 inPublished:November 11, 2004Publisher:Cambridge University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0521611997

ISBN - 13:9780521611992


Table of Contents

Acknowledgements; 1. Introduction; 2. Fluid flow dynamics; 3. Light and optics; 4. Electronics; 5. Computing; 6. Cell sorting; 7. Preparation and staining; 8. Miscellaneous techniques; 9. Instrument performance; 10. Light scatter applications; 11. Nucleic acid analysis; 12. Nucleic acids and protein; 13. Chromosomes; 14. Dynamic cellular events; 15. Applications in oncology; 16. Epilogue; References; Index.

Editorial Reviews

..".a highly individualistic view of flow cytometry and as such is not only informative but is a 'good read'....has succeeded in producing a book which is more than just an introduction to flow cytometry. People reading this book will benefit from the author's experience, knowledge and foresight to help them in their own application. I thoroughly recommend this book to anyone who has an interest in flow cytometry." George D. Wilson, BioEssays