Ion Channel Localization by Anatoli LopatinIon Channel Localization by Anatoli Lopatin

Ion Channel Localization

EditorAnatoli Lopatin, Colin G. Nichols

Paperback | November 9, 2010

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Experienced researchers at the cutting-edge of their fields describe how to use current and developing technologies to determine the exact location of ion channels and receptors in cells and cell membranes. With emphasis on techniques employing the unique properties of these proteins, the core topics of the book include all the major pharmacological tools for ion channel determination. Also covered are fluorescent and radioligand assays, exciting new visualization methods employing green fluorescent protein (GFP), and novel assays based on the functional properties of ion channels and receptors, as well as rapidly developing atomic force microscopy methodologies. Comprehensive and rich in practical detail, Ion Channel Localization Methods and Protocols brings together in a single volume detailed reviews of all the major techniques-both currently in use and in development-for localizing ion channels and receptors in cells, tissues, and organisms.
Title:Ion Channel LocalizationFormat:PaperbackDimensions:520 pages, 9.02 × 5.98 × 0.04 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372110

ISBN - 13:9781617372117

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Table of Contents

Part I. Pharmacological Labeling of Ion Channels and ReceptorsFluorescent Calcium Antagonists: Tools for Imaging of L-Type Calcium Channels in Living CellsThomas BuddeTargeting Cerebral Muscarinic Acetylcholine Receptors with Radioligands for Diagnostic Nuclear Medicine StudiesDaniel W. McPhersonGABAA Receptors: Autoradiographic Localization of Different Ligand Sites and Identification of Subtypes of the Benzodiazepine Binding SiteCyrille Sur and John AtackLocalization of ATP P2X ReceptorsXuenong Bo and Geoffrey BurnstockSmall Conductance Calcium-Activated Potassium Channels in Rat Brain: Autoradiographic Localization Using Two Specific Toxins, Apamin and ScyllatoxinMarc BorsottoMapping N-Type Calcium Channel Distributions with _-ConotoxinsGeula M. Bernstein and Owen T. JonesFluorescent Imaging of Nicotinic Receptors During Neuromuscular Junction DevelopmentZhengshan Dai and H. Benjamin PengHigh Affinity Scorpion Toxins for Studying Potassium and Sodium ChannelsLourival D. Possani, Baltazar Becerril, Jan Tytgat, and Muriel DelepierrePart II. Applications of Green Fluorescent Protein (GFP) TechnologyBicistronic GFP Expression Vectors as a Tool to Study Ion Channels in Transiently Transfected Cultured CellsJan Eggermont, Dominique Trouet, Gunnar Buyse, Rudi Vennekens, Guy Droogmans, and Bernd NiliusConfocal Imaging of GFP-Labeled Ion ChannelsBryan D. Moyer and Bruce A. StantonLocalization and Quantification of GFP-Tagged Ion Channels Expressed in Xenopus OocytesTooraj Mirshahi, Diomedes E. Logothetis, and Massimo SassaroliApplications of Green Fluorescent Protein (GFP) Technology: Watching Ion Channel Biogenesis in Living Cells Using GFP Fusion ConstructsScott A. John and James N. WeissTagging Ion Channels with the Green Fluorescent Protein (GFP) as a Method for Studying Ion Channel Function in Transgenic Mouse ModelsJoseph C. Koster and Colin G. NicholsMutant GFP-Based FRET Analysis of K+ Channel OrganizationElena N. Makhina and Colin G. NicholsDelivering Ion Channels to Mammalian Cells by Membrane FusionDavid C. Johns, Uta C. Hoppe, Eduardo Marbán, and Brian O'RourkePart III. Functional Assays in Ion Channel LocalizationDetection of Neurons Expressing Calcium-Permeable AMPA Receptors Using Kainate-Induced Cobalt UptakeCristóvão Albuquerque, Holly S. Engelman, C. Justin Lee, and Amy B. MacDermottCrystallization Technique for Localizing Ion Channels in Living CellsAnatoli N. Lopatin, Elena N. Makhina and Colin G. NicholsImaging of Localized Neuronal Calcium InfluxFritjof HelmchenUsing Caged Compounds to Map Functional Neurotransmitter ReceptorsDiana L. Pettit and George J. AugustinePart IV. Atomic Force MicroscopyAtomic Force Microscopy of Reconstituted Ion ChannelsHoeon Kim, Hai Lin, and Ratneshwar LalImaging the Spatial Organization of Calcium Channels in Nerve Terminals Using Atomic Force MicroscopyHajime Takano, Marc Porter, and Philip G. HaydonNanoarchitecture of Plasma Membrane Visualized with Atomic Force MicroscopyHans Oberleithner, Hermann Schillers, Stefan W. Schneider, and Robert M. HendersonDynamic AFM of Patch Clamped MembranesKenneth Snyder, Ping C. Zhang, and Frederick SachsLocalizing Ion Channels with Scanning Probe Microscopes: A PerspectiveDaniel M. Czajkowsky and Zhifeng Shao

Editorial Reviews

"There is a nice balance of review and methodology, with an emphasis on methodological principles. The scope of the book provides a rich and comprehensive review of staid and state-of-the-art localization techniques and their rationales for use. This is a singular and needed addition to the field of ion channel physiology, cell biology and neurobiology"-Doody's Health Sciences Book Review Journal"...both timely and will be of considerable interest to researchers of ion channel function....This book represents a collection of 24 methods of ion channel localization written by leading specialists and divided into four main parts. The first part concentrates on the fluorescent and radioligand binding of channel or receptors with specific pharmacological agents or toxins....Thus, the second part of this book concentrates on the quantification and cellular or subcellular visualization of ion channel expression in oocytes and immortal cell lines using green fluorescent proteins (GFP)....The third section of Ion Channel Localization: Methods and Protocols utilizes the functional properties of the channels for localization....One of the major achievements of Ion Channel Localization: Methods and Protocols in gathering so many of today's technologies of channel visualization is the candid and detailed descriptions the authors have provided of the advantages, pitfalls and 'tricks of the trade' of the methods under discussion. Descriptions are such I am confident that many scientists will be tempted to have a go themselves." - Clinical and Experimental Pharmacology and Physiology