Laboratory Methods in Cell Biology: Biochemistry and Cell Culture

Other | December 31, 2012

byP. Michael Conn, P. Michael Conn

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Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume ofMethods in Cell Biologycovers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation.



  • Covers the most important laboratory methods in cell biology
  • Chapters written by experts in their fields

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Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume ofMethods in Cell Biologycovers laboratory methods in cell biology, and includes methods that are among the most...

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and O...

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Format:OtherDimensions:544 pages, 1 × 1 × 1 inPublished:December 31, 2012Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0124055494

ISBN - 13:9780124055490

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Table of Contents

  1. Extraction/Characterization of DNA
  2. Immunoprecipitation for Isolation of Protein Signaling Complexes from Native Tissues
  3. Realtime PCR
  4. Single Cell Gel Electrophoresis
  5. High Throughput Methods for the Identification of Protein Purification Conditions Using a Cleavable Tag System
  6. Difference Gel Electrophoresis
  7. Optimization of Cell Cycle Measurement by Time Lapse-Microscopy
  8. Transfection
  9. Cell Enrichment
  10. Magnetic Batch Separation
  11. Lateral Flow Assays
  12. Measurement of Cell Regeneration
  13. DNA Damage Analysis in Cells
  14. Purification of Cardiac Cells
  15. Endothelial Cell Tube Formation in vitro assays
  16. Single-particle Analysis
  17. Measurement of Cell Invasivness
  18. Techniques for Cell Sorting
  19. Stem Cells for Drug Discovery
  20. Quantifying Apoptosis in 96-well Plates
  21. Pulse Cytometry
  22. Cell Motility
  23. Single Cell Deposition
  24. Enzyme Sensors for Living Cells
  25. Myoblast purifying by magnetic-activated cell sorting (MACS)