Laboratory Methods In Enzymology: Protein Part C by Jon Lorsch

Laboratory Methods In Enzymology: Protein Part C

byJon LorschEditorJon Lorsch

Hardcover | April 10, 2014

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In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.



  • Indispensable tool for the researcher
  • Carefully written and edited by experts to contain step-by-step protocols
  • In this volume we have brought together a number of core protocols concentrating on Protein

Details & Specs

Title:Laboratory Methods In Enzymology: Protein Part CFormat:HardcoverDimensions:296 pages, 9.41 × 7.24 × 0.98 inPublished:April 10, 2014Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0124201199

ISBN - 13:9780124201194

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Table of Contents

TCA Precipitation Coimmunoprecipitation of Proteins from Yeast Coupling Antibody to Cyanogen Bromide-Activated Sepharose Analysis of Protein Protein Interactions by Coimmunoprecipitation Use and Application of Hydrophobic Interaction Chromatography for Protein Purification Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins Salting out of Proteins Using Ammonium Sulfate Precipitation Using Ion Exchange Chromatography to Purify a Recombinantly Expressed Protein Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins Expression and Purification of Membrane Proteins Explanatory Chapter: Choosing the Right Detergent One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE) Coomassie Blue Staining Silver Staining of SDS-polyacrylamide Gel Standard In Vitro Assays for Protein Nucleic Acid Interactions Gel shift Assays for RNA and DNA Binding Protein Filter Binding Explanatory Chapter: Troubleshooting recombinant protein expression: general Explanatory Chapter: Troubleshooting protein expression: what to do when the protein is not soluble Western Blotting using Chemiluminescent Substrates