Laboratory Methods In Enzymology: Protein Part B

Hardcover | March 11, 2014

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Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on protein

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Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols...

Format:HardcoverDimensions:240 pages, 9.41 × 7.24 × 0.98 inPublished:March 11, 2014Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0124201202

ISBN - 13:9780124201200

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Table of Contents

Small-scale expression of proteins inE. coliProtein expression-Yeast Recombinant protein expression in baculovirus-infected insect cells Single cell cloning of a stable mammalian cell line In vitro synthesis of proteins in bacterial extracts Preparation of aSaccharomyces cerevisiaecell-free extract for in vitro translation Yeast two-hybrid screen UV cross-linking of interacting RNA and protein in cultured cells Analysis of RNA protein interactions by cell mixing General protein protein cross-linking Chromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematodeCaenorhabditis elegansPAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteins Determining the RNA specificity and targets of RNA-binding proteins using a three-hybrid system Dissecting a known RNA protein interaction using a yeast three-hybrid system Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system