Laser Capture In Microscopy And Microdissection: Methods In Enzymology

Other | November 1, 2002

byConn, P. Michael, P. Michael Conn

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The critically acclaimed laboratory standard for more than forty years,Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
  • Basic Principles, Specialized Uses, and Genetic Applications
  • LCM and its application in genomics and proteomics
  • Fluorescence in situ hybridization of LCM isolated nuclei from paraffin sections
  • Noncontact laser catapulting for the functional genomics and proteomics
  • Use of LCM for clonal analysis, in carcinoma analysis, to assess development, in complex tissue, in pathology, gene discovery, and more

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The critically acclaimed laboratory standard for more than forty years,Methods in Enzymologyis one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them st...

P. Michael Conn is the Senior Vice President for Research and Associate Provost, Texas Tech Health Sciences Center. He is The Robert C. Kimbrough, Professor of Internal Medicine and Cell Biology/Biochemistry. He was previously Director of Research Advocacy and Professor of Physiology and Pharmacology, Cell Biology and Development and O...

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Format:OtherDimensions:775 pages, 1 × 1 × 1 inPublished:November 1, 2002Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080497012

ISBN - 13:9780080497013

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Table of Contents

Comparison of Current Equipment
Laser Capture Microdissection (LCMD) and its Applications in Genomics & Proteomics
Going in vivo With Laser MD (LMD)
Use of LCMD to Selectively Obtain Distinct Populations of Cells for Proteomic Analysis
Optimized Tissue Processing and Staining for LCMD and Nucleic Acid Retrieval
Fluoroscence in situ Hybridization of LCM-isolated Nuclei from Paraffin Sections
Immunoblotting of Single Cell Types Isolated from Frozen Sections by LMD
Noncontact Laser Catapulting: A Basic Procedure for Functl' Genomics and Proteomics
Internal Standards for LMD
Methacarn: A Fixation Tool for Multipurpose Genetic Analysis from Paraffin-Embedded Tissues
Use of LCMD for Clonal Analysis
LCMD in Carcinoma Analysis
LCMD to Assess Devt
Application of LCMD to Proteomics
LCMD of the Mouse Intestine: Characterizing mRNA and Protein Expression, and Profiling Intermediary Metabolism in Specified.
LCMD in Pathology
Use of Laser Capture Microscopy (LCM) in the Analysis of Mouse Models of Human Diseases
Use of LMD in Complex Tissue
Assessmt of Clonal Relationships in Malignant Melanomas
Comp. of Normal and Tumor Cells by LCMD
Analysis of Folliculostellate Cells by LCMD and Reverse Transcription-Polymerase Chain Reaction (LCM-RT/PCR)
Analysis of Gene Expression
Analysis of Specific Gene Expression
Gene Discovery With LCM
DNA Fingerprinting from Cells Captured by LMD
Single Cell PCR in LCM
Assessmt of Genetic Clonality in Tumors using LCMD
Gene Mutations: Analysis in Proliferative Prostatic Diseases Using LCMD
Use of LCMD Generated Targets for Hybridization of High-Density Oligonucleotide Arrays. Single Cell Gene Mutation Analysis Using Laser-Assisted MD of Tissue Sections
Methylation in Gene Promoters: Assessmt after LCMD