Liposome Methods and Protocols by Subhash C. BasuLiposome Methods and Protocols by Subhash C. Basu

Liposome Methods and Protocols

EditorSubhash C. Basu, Manju Basu

Paperback | November 10, 2010

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A panel of skilled researchers describes in step-by-step detail their best laboratory techniques for elucidating liposome biology. With these readily reproducible methods, investigators can illuminate such critical questions as the attachment of liposmes to cell surfaces, the permeation of liposomes through the plasma membranes, and the stability of liposomes in cellular and nuclear matrices. Each detailed protocol is presented by an investigator who is intimately familiar with its problems and capabilities, and includes a detailed list of materials to be used, troubleshooting tips straight from the benchtop, and notes on pitfalls to avoid.
Title:Liposome Methods and ProtocolsFormat:PaperbackDimensions:264 pages, 9.02 × 5.98 × 0.07 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737217X

ISBN - 13:9781617372179

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Table of Contents

Part I. Physical PropertiesPreparation, Isolation, and Characterization of Liposomes Containing Natural and Synthetic LipidsSubroto Chatterjee and Dipak K. BanerjeePreparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model SystemsMassimo Masserini, Paola Palestini, Marina Pitto, Vanna Chigorno, and Sandro SonninoPart II. Liposome Fusion/ModulationPeptide-Induced Fusion of LiposomesEve-Isabelle Pécheur and Dick HoekstraLiposomes: Applications in Protein-Lipid Interaction Studies,Sujoy Ghosh and Robert BellLipids in Viral FusionAnu Puri, Maite Paternostre, and Robert BlumenthalPart III. Application of LiposomesLiposome-Mediated, Fluorescence-Based Studies of Sphingolipid Metabolism in Intact CellsShimon Gatt, Tama Dinur, and Arie DaganMicelles and Liposomes in Metabolic Enzyme and Glycolipid Glycosyltransferase AssaysManju Basu and Subhash BasuLiposomes and Phospholipid Binding Proteins in Glycoprotein BiosynthesisRoger K. Bretthauer and Dennis W. WelshTherapeutic Uses of Antioxidant LiposomesWilliam L. Stone, Shyamali Mukherjee, Milton Smith, and Salil K. DasTargeted Gene Delivery by VirosomesDebi P. Sarkar, Komal Ramani, and Sandeep K. TyagiLiposomes Containing Ligands: Binding Specificity to SelectinsSriram Neelamegham and Khushi L. MattaPreparation and Characterization of Glycolipid-Bearing Multilamellar and Unilamellar LiposomesP. R. Satish and A. SuroliaUse of Liposomes Containing Carbohydrates for Production of Monoclonal AntibodiesReiji KannagiInhibition of Tumor Metastasis by Liposomes Containing Glyco-Replica PeptidesTakao Taki and Naoto OkuUse of Phospholipid Bilayers and Monolayers in Binding Studies of Vitamin K-Dependent Blood Coagulation ProteinsFrancis J. Castellino and Eric H. EllisonIndex

Editorial Reviews

"...those who deal with liposome in their work, this book with detailed laboratory protocols and many notes will be very useful." -Pharmaceutical Research"Volume 199 of the series Methods in Molecular BiologyT, offers an excellent overview of protocols used for the study of liposomes in biology...the book portrays comprehensive reports of the study of the cellular fate of sphingolipids using fluoresrole lipids play in virus fusion, studies of functions of various membrane-associated proteins, specifically of phospholipid binding proteins in glycoprotein biosynthesis and contributions of therapeutical liposome applications." -Molecular Biotechnology