Liposomes: Methods and Protocols, Volume 2: Biological Membrane Models by Volkmar WeissigLiposomes: Methods and Protocols, Volume 2: Biological Membrane Models by Volkmar Weissig

Liposomes: Methods and Protocols, Volume 2: Biological Membrane Models

EditorVolkmar Weissig

Paperback | April 6, 2012

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With nearly one hundred years of intensive study, lipids have proven to be a vital and ever-more-promising area of cell biological research. In Liposomes: Methods and Protocols, leading experts in the related fields explore cutting-edge experimental methods involving all aspects of lipids as essential components of the cell membrane. Volume 2: Biological Membrane Models focuses on detailed methods for the use of liposomes in studying a variety of biochemical and biophysical membrane phenomena concomitant with chapters describing a great palette of state-of-the-art analytical technologies. As a volume in the highly successful Methods in Molecular BiologyT series, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and authoritative, Liposomes: Methods and Protocols promises to be an essential source of practical know-how for every investigator, young and seasoned alike, whose research area involves in one way or another phospholipids, glycolipids, or cholesterol.
Title:Liposomes: Methods and Protocols, Volume 2: Biological Membrane ModelsFormat:PaperbackDimensions:564 pages, 10 × 7.01 × 0.03 inPublished:April 6, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617796875

ISBN - 13:9781617796876

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Table of Contents

1. Utilization of Liposomes for Studying Drug Transfer and UptakeAlfred Fahr and Xiangli Liu2. The Use of Liposomes in the Study of Drug Metabolism: A Method to Incorporate the Enzymes of the Cytochrome P450 Monooxygenase System into Phospholipid, Bilayer VesiclesJames R. Reed3. Use of Liposomes to Study Cellular OsmosensorsReinhard Krämer, Sascha Nicklisch, and Vera Ott4. Studying Mechanosensitive Ion Channels Using LiposomesBoris Martinac, Paul R. Rohde, Andrew R. Battle, Evgeny Petrov, Prithwish Pal, Alexander Fook Weng Foo, Valeria Vásquez, Thuan Huynh, and Anna Kloda5. Studying Amino Acid Transport Using LiposomesCesare Indiveri6. Use of Liposomes for Studying Interactions of Soluble Proteins with Cellular MembranesChris Höfer, Andreas Herrmann, and Peter Müller7. Liposomal Reconstitution of Monotopic Integral Membrane ProteinsZahra MirAfzali and David L. DeWitt8. The Reconstitution of Actin Polymerization on LiposomesMark Stamnes and Weidong Xu9. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains Under Physiological Ionic-Strength ConditionsL.-Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis A. Bagatolli, and Felix M. Goñi10. Visualization of Lipid Domain Specific Protein Sorting in Giant Unilamellar VesiclesMartin Stöckl, Jörg Nikolaus, and Andreas Herrmann11. Biosynthesis of Proteins Inside LiposomesPasquale Stano, Yutetsu Kuruma, Tereza Pereira de Souza, and Pier Luigi Luisi12. Study of Respiratory Cytochromes in LiposomesIseli L. Nantes, Cintia Kawai, Felipe S. Pessoto, and Katia C.U. Mugnol13. Use of Liposomes to Evaluate the Role of Membrane Interactions on Antioxidant ActivitySalette Reis, Marlene Lúcio, Marcela Segundo, and José L.F.C. Lima14. Studying Colloidal Aggregation Using LiposomesJuan Sabín, Gerardo Prieto, Juan M. Ruso, and Félix Sarmiento15. Assessment of Liposome-Cell InteractionsJan A.A.M. Kamps16. Methods to Monitor Liposome Fusion, Permeability, and Interaction with CellsNejat Düzgünes, Henrique Faneca, and Maria C. Pedroso de Lima17. The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in LiposomesDavid Miller and Paula J. Booth18. Studying Lipid Organization in Biological Membranes Using Liposomes and EPR Spin LabelingWitold K. Subczynski, Marija Raguz, and Justyna Widomska19. Membrane Translocation Assayed by Fluorescence SpectroscopyJana Broecker and Sandro Keller20. Interaction of Lipids and Ligands with Nicotinic Acetylcholine Receptor Vesicles Assessed by Electron Paramagnetic Resonance SpectroscopyHugo Rubén Arias21. Environmental Scanning Electron Microscope Imaging of Vesicle SystemsYvonne Perrie, Habib Ali, Daniel J. Kirby, Afzal U.R. Mohammed, Sarah E. McNeil, and Anil Vangala22. Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution ScaleBrigitte Papahadjopoulos-Sternberg23. Atomic Force Microscopy for the Characterization of ProteoliposomesJohannes Sitterberg, Maria Manuela Gaspar, Carsten Ehrhardt, and Udo Bakowsky24. Method of Simultaneous Analysis of Liposome Components Using HPTLC/FIDSophia Hatziantoniou and Costas Demetzos25. Viscometric Analysis of DNA-Lipid ComplexesSadao Hirota and Nejat Düzgünes26. Fluorometric Analysis of Individual Cationic Lipid-DNA ComplexesEdwin Pozharski27. Fluorescence Resonance Energy Transfer (FRET) Based Analysis of LipoplexesEdwin Pozharski28. Analysis of Lipoplex Structure and Lipid Phase ChangesRumiana Koynova-Tenchova29. Fluorescence Methods for Evaluating Lipoplex-Mediated Gene DeliveryHenrique Faneca, Nejat Düzgünes, and Maria C. Pedroso de Lima30. FRET Imaging of Cells Transfected with siRNA/Liposome ComplexesIl-Han Kim, Anne Järve, Markus Hirsch, Roger Fischer, Michael F. Trendelenburg, Ulrich Massing, Karl Rohr, and Mark Helm31. Spectral Bio-Imaging and Confocal Imaging of the Intracellular Distribution of LipoplexesSebastian Schneider and Regine Peschka-Süss32. Techniques for Loading Technetium-99m and Rhenium-186/188 Radionuclides into Preformed Liposomes for Diagnostic Imaging and Radionuclide TherapyBeth Goins, Ande Bao, and William T. Phillips33. Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant Unilamellar VesiclesAna J. García-Sáez, Dolores C. Carrer, and Petra Schwille34. Liposome Biodistribution via Europium ComplexesNathalie Mignet and Daniel Scherman35. Biosensor-Based Evaluation of Liposomal Binding BehaviorGerd Bendas36. Use of Liposomes to Study Vesicular TransportKohji Takei, Hiroshi Yamada, and Tadashi Abe

Editorial Reviews

From the reviews:"Provide a comprehensive guide to the preparation, characterisation, and potential use of currently known types of liposomes. They are written using an interdisciplinary approach, and so provide essential knowledge of liposomes, for not only researchers in this particular field, but also scientists in wider pharmaceutical and biological areas. . very inspiring - the potential for liposomes is immense - and these well-written chapters ignited notions of further applications - this is clearly a very exciting field." (Trudy L. Knight, BTS Newsletter, Issue 37, Winter, 2010)