Lotus japonicus Handbook by Antonio J. MárquezLotus japonicus Handbook by Antonio J. Márquez

Lotus japonicus Handbook

EditorAntonio J. Márquez, J. Stougaard, M. Udvardi

Paperback | October 19, 2010

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Legumes are very important plants playing a central role in biological research. They are a key component of sustainable agricultural systems because of symbiotic nitrogen fixation and other beneficial symbiosis with mycorrhizal fungi. Studies on most of the major leguminous crops are hampered by large genome sizes and other disadvantages which have hindered the isolation and characterisation of genes with important roles in legume biology and agriculture. For this reason Lotus japonicus was chosen as a model species for legume research some ten years ago. Since then, many groups around the world have adopted Lotus as a model and have developed numerous resources and protocols to facilitate basic and applied research on this species. This handbook represents the first effort to compile basic descriptions and methods for research in Lotus, including symbiotic processes, cell and molecular biology protocols, functional genomics, mutants, gene tagging and genetic analysis, transformation and reverse genetic analysis, primary and secondary metabolism, and an exhaustive update of the scientific literature available on this plant.
Title:Lotus japonicus HandbookFormat:PaperbackDimensions:406 pages, 9.45 × 6.3 × 0.07 inPublished:October 19, 2010Publisher:Springer NetherlandsLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:9048169453

ISBN - 13:9789048169450

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Table of Contents

ContentsList of editors Preface Colour plates 1 Lotus japonicus, a general introductionJ. Stougaard and A.J. Márquez 1.1 Lotus japonicus as a model system E. Pajuelo and J. Stougaard1.2 Lotus-related species and their agronomic importance P. Díaz, O. Borsani, and J. Monza1.3 Drought and saline stress P. Díaz, J. Monza, and A.J. MárquezSymbiotic processes M. Udvardi and M. Parniske 2.1 Methods for studying nodule development and function M. Hayashi, M.L. Tansengco, N. Suganuma, K. Szczyglowski, L. Krusell, T. Ott, and M. Udvardi2.2 A procedure for in vitro nodulation studies A. Barbulova and M. Chiurazzi2.3 Arbuscular mycorrhiza S. Kosuta, T. Winzer, and M. Parniske3 Cell and molecular biology protocolsH.P. Spaink and M. Parniske 3.1 Concurrent visualization of gusA and lacZ reporter gene expression C. Díaz, H.R.M. Schlaman, and H.P. Spaink3.2 Embedding root and nodule tissue in plastic (BMM) M. GrÆ nlund, A. Agalou, M.C. Rubio, G.E.M. Lamers, A. Roussis, and H.P. Spaink3.3 RNA isolation using phase extraction and LiCl precipitation C. Kistner and M. Matamoros3.4 RNA isolation using CsCl gradients T. Ott, L. Krussell, and M. Udvardi3.5 96-well DNA isolation method J. Perry and M. Parniske4 Functional genomicsM. Udvardi and G. Saalbach 4.1 Genome structural analysis S. Tabata4.2 Transcriptome analysis using cDNA arrays T. Ott, L. Krusell, S. Freund, and M. Udvardi4.3 Lotus japonicus expression database S. Kloska, P. Krüger, and J. Selbig4.4 Procedures for mass spectrometric proteome analysis G. Saalbach4.5 Isolation of peribacteroid membranes for proteome analysis S. Wienkoop4.6 Metabolome analysis using GC-MS G. Debrosses, D. Steinhauser, J. Kopka, and M. UdvardiMutants, gene-tagging, and genetic analysis J. Stougaard and K.J.Webb 5.1 Mutagenesis K.J. Webb, M. Robbins, T.L. Wang, M. Parniske, and A.J. Márquez5.2 A mutant catalogue of Lotus japonicus E. Pajuelo 5.3 TILLING J. Perry, T. Welham, S. Cheminant, M. Parniske, and T. Wang5.4 Ds gene tagging L. Tirichine, J.A. Herrera-Cervera, and J. Stougaard5.5 Mapping and map-based cloning N. Sandal, K. Krause, M. Frantescu, S. Radutoiu, L. Krusell, L.H. Madsen, N. Kanamori, E. Madsen, L. Tirichine, and J. Stougaard5.6 An in silico strategy using comparative sequence analysis L. Schauser, S. Subrahmanyam, L.H. Madsen, N. Sandal, and J. Stougaard5.7 Information transfer: mapping and cloning in other legumes L.H. Madsen and J. Stougaard6 Transformation and reverse genetic analysisM. Chiurazzi 6.1 Agrobacterium-mediated in vitro transformation P. Lombari, E. Ercolano, H. El Alaoui, and M. Chiurazzi6.2 Induction of hairy roots for symbiotic gene expression studies C.L. Diaz, M. Gronlund, H.R.M. Schlaman, and H.P. Spaink6.3 Transformation-regeneration procedure L. Tirichine, J.A. Herrera, and J. Stougaard6.4 Agrobacterium rhizogenes pRi TL-DNA integration system S. Radutoiu, L.H. Madsen, E.B. Madsen, and J. Stougaard6.5 Vectors for reverse genetics and expression analysis S.U. Andersen, C. Cvitanich, M. Gronlund, H. Busk, D.B. Jensen, and E.O. Jensen7 Primary and secondary metabolismA.J. Marquez and K.J. Webb 7.1 Nitrate assimilation: Influence of nitrogen supply A. Orea, P. Pajuelo, J.M. Romero, and A.J. Márquez7.2 Nitrate and ammonium assimilatory enzymes A.J. Márquez, M. Betti, M. García-Calderón, G. Estivill, A. Credali, P. Pajuelo, A.Orea, M.T. Clemente, E. Pajuelo, F. Galván 7.3 Transgenic plants affected in nitrate assimilation A. Orea, I. Prosser, J.M. Romero, and A.J. Márquez7.4 Secondary metabolite profiling N. Rispail, R. Nash, and K.J. Webb7.5 Phenolic compounds: extraction and analysis N. Rispail, P. Morris, and K.J. Webb7.6 Elicitation of isoflavan phytoalexins A. Lanot and P. Morris8 Lotus japonicus literatureM. Betti and A.J. Marquez 8.1 An update of work published on Lotus japonicus M. Betti and A.J. Márquez Index