Lymphocytes: A Practical Approach

Paperback | December 16, 1999

EditorSarah L. Rowland-Jones, Andrew Mcmichael

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Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy.Many investigators have been discourages by the complicated protocols involved in cellular immunological studies, as illustrated, by the meticulous care required for the generation of antigen-specific T-cells. Lymphocytes: A Practical Approach (second edition) contains straight-forward protocolsfor well- established procedures in the study of lymphocytes including preparation and identification of lymphocytes, immortalization, cell and organ culture, and quantification assays. It also covers the recent technological advances which have revolutionised the field, such as the use of theInterferon-gamma ELISpot assay and peptide-HLA tetrameric assays to quantify antigen-specifidc T-cells directly from peripheral blood, without the need for in vitro culture, and molecular methods for accurate HLA typing.

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Cellular immunology is a rapidly moving field in which recent advances have made significant contributions to our understanding of the immune response to infection and malignancy. These in turn, have given rise to new therapeutic opportunities in areas such as vaccines and immunotheraphy.Many investigators have been discourages by the...

Sarah L. Rowland-Jones is at Institute of Molecular Medicine, Oxford. Andrew McMichael is at Institute of Molecular Medicine, Oxford.

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Format:PaperbackPublished:December 16, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638160

ISBN - 13:9780199638161

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Table of Contents

PrefacePlebanski: Preparation of lymphocytes and idenfication of lymphocyte subpopulationsBiddulph and Gatter: Immunohistochemistry of lymphoid organsMeinl and Fickenscher: Bastin: T and B-cell hybridomasMills: Murine T-cell cultureVyakarnam: Human CD4+ T-cell cultureRowland-Jones: Human Cytotoxic T-cell cultureCarmichael: Limiting dilution analysis for quantification of antigen-specific T-cellsOgg: HLA -peptide tetrameric complexesTan: Expansion of human T-cells for immunotheraphyBrowning and Krausa: HLA typingBrown and Symons: Characterisation of lymphocyte surface markersBangham and Furukawa: Apoptosis assays for lymphocytesOwen and McLoughlin: Thymic organ cultureIndex

Editorial Reviews

"... the book contains extremly useful practical advice for studying lymphocytes in the laboratory..." Parasitology Today