Lymphoma: Methods and Protocols by Ralf KüppersLymphoma: Methods and Protocols by Ralf Küppers

Lymphoma: Methods and Protocols

EditorRalf Küppers

Hardcover | January 8, 2013

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Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research.  Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.

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Title:Lymphoma: Methods and ProtocolsFormat:HardcoverDimensions:326 pages, 10 × 7.01 × 0.1 inPublished:January 8, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627032681

ISBN - 13:9781627032681

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Table of Contents

1. Origin and Pathogenesis of B Cell Lymphomas            Marc Seifert, René Scholtysik, and Ralf Küppers 2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma            David Wu, Brent L. Wood, and Jonathan R. Fromm 3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells            Ralf Küppers, Markus Schneider, and Martin-Leo Hansmann 4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics            Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group 5. Expression Cloning of Human B Cell Immunoglobulins            Hedda Wardemann and Juliane Kofer 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR            Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen 7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies            Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas            Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas            Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group 5. Expression Cloning of Human B Cell Immunoglobulins            Hedda Wardemann and Juliane Kofer 6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR            Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen 7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies            Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas            Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Martin J.S. Dyer 8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas            Nikos Darzentas and Kostas Stamatopoulos 9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba 10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba 11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass 12. Gene Expression Profile Analysis of Lymphomas            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Katia Basso and Ulf Klein 13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Maciej Gie?ng and Reiner Siebert 14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens            Vu N. Ngo 15. Studying MicroRNAs in Lymphoma            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg 16. Molecular Methods of Virus Detection in Lymphoma            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer 17. High Throughput RNA Sequencing in B-Cell Lymphomas            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz