Lymphoma: Methods and Protocols by Ralf KüppersLymphoma: Methods and Protocols by Ralf Küppers

Lymphoma: Methods and Protocols

byRalf Küppers

Hardcover | January 8, 2013

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Lymphomas are lymphoid malignancies derived from B or T lymphocytes, and their study has been and still is paradigmatic for many aspects of cancer research.  Lymphoma: Methods and Protocols presents and discusses key methods that are used in lymphoma research, partly specific for lymphoma research but often adaptable to the study of other cancers. By covering a broad variety of methods used in lymphoma research, this book will be of interest not only for hematologists, hematopathologists, and immunologists but also for scientists interested in other fields of cancer research as well as human genetics. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Versatile and cutting-edge, Lymphoma: Methods and Protocols serves researchers studying human physiology with the ultimate goal of understanding and controlling these often terrible diseases.
Title:Lymphoma: Methods and ProtocolsFormat:HardcoverDimensions:315 pagesPublished:January 8, 2013Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627032681

ISBN - 13:9781627032681

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Table of Contents

1. Origin and Pathogenesis of B Cell Lymphomas

            Marc Seifert, René Scholtysik, and Ralf Küppers

 

2. Flow Cytometry for Non-Hodgkin and Classical Hodgkin Lymphoma

            David Wu, Brent L. Wood, and Jonathan R. Fromm

 

3. Laser-Based Microdissection of Single Cells from Tissue Sections and PCR Analysis of Rearranged Immunoglobulin Genes from Isolated Normal and Malignant Human B Cells

            Ralf Küppers, Markus Schneider, and Martin-Leo Hansmann

 

4. PCR-Based Analysis of Rearranged Immunoglobulin or T-Cell Receptor Genes by GeneScan Analysis or Heteroduplex Analysis for Clonality Assessment in Lymphoma Diagnostics

            Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group

 

5. Expression Cloning of Human B Cell Immunoglobulins

            Hedda Wardemann and Juliane Kofer

 

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

            Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen

 

7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

            Martin J.S. Dyer

 

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

            Nikos Darzentas and Kostas Stamatopoulos

 

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba

 

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

 

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass

 

12. Gene Expression Profile Analysis of Lymphomas

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Martin J.S. Dyer

 

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

            Nikos Darzentas and Kostas Stamatopoulos

 

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba

 

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

 

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass

 

12. Gene Expression Profile Analysis of Lymphomas

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Elke Boone, Brenda Verhaaf, and Anton W. Langerak, on behalf of the EuroClonality / BIOMED-2 Collaborative Study Group

 

5. Expression Cloning of Human B Cell Immunoglobulins

            Hedda Wardemann and Juliane Kofer

 

6. Studying the Replication History of Human B Lymphocytes by Real-Time Quantitative (RQ)-PCR

            Menno C. van Zelm, Magdalena A. Berkowska, and Jacques J.M. van Dongen

 

7. The Detection of Chromosomal Translocations Involving the Immunoglobulin Loci in B-Cell Malignancies

            Martin J.S. Dyer

 

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

            Nikos Darzentas and Kostas Stamatopoulos

 

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba

 

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

 

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass

 

12. Gene Expression Profile Analysis of Lymphomas

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Martin J.S. Dyer

 

8. Stereotyped B Cell Receptors in B Cell Leukemias and Lymphomas

            Nikos Darzentas and Kostas Stamatopoulos

 

9. Flow Cytometric MRD Detection in Selected Mature B-Cell Malignancies

            Sebastian Böttcher, Matthias Ritgen, and Michael Kneba

 

10. MRD Detection in B-Cell Non-Hodgkin Lymphomas Using Ig Gene Rearrangements and Chromosomal Translocations as Targets for Real-Time Quantitative PCR

            Christiane Pott, Monika Brüggemann, Matthias Ritgen, Vincent H.J. van der Velden, Jacques J.M. van Dongen, and Michael Kneba

 

11. Enrichment of Methylated DNA by Methyl-CpG Immunoprecipitation

            Miriam Sonnet, Constance Bär, Michael Rehli, Dieter Weichenhan, and Christoph Plass

 

12. Gene Expression Profile Analysis of Lymphomas

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Katia Basso and Ulf Klein

 

13. FISH and FICTION to Detect Chromosomal Aberrations in Lymphomas

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Maciej Gie?ng and Reiner Siebert

 

14. Identification of Pathogenetically Relevant Genes in Lymphomagenesis by shRNA Library Screens

            Vu N. Ngo

 

15. Studying MicroRNAs in Lymphoma

            Joost Kluiver, Izabella Slezak-Prochazka, and Anke van den Berg

 

16. Molecular Methods of Virus Detection in Lymphoma

            Ruth F. Jarrett, Alice Gallagher, and Derek Gatherer

 

17. High Throughput RNA Sequencing in B-Cell Lymphomas

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz

            Wenming Xiao, Bao Tran, Louis M. Staudt, and Roland Schmitz