Macromolecular Crystallization and Crystal Perfection

Hardcover | May 1, 2010

byNaomi E. Chayen, John R. Helliwell, Edward H. Snell

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The crystallization of proteins and nucleic acids and/or their complexes has become more highly automated but is still often a trial and error based approach. In parallel, a number of X-ray diffraction based techniques have been developed which explain the physical reasons limiting theresulting crystallographic data and thus show how that data may be improved. Crystal growth is also pivotal in neutron crystallography, which establishes the hydrogen and hydration aspects. Thus this book is aimed at addressing the science behind obtaining the best and most complete structural datapossible for biological macromolecules, so that the detailed structural biology and chemistry of these important molecules emerge. Crystal imperfections such as twinning and lattice disorders, as well as multiple crystal situations, and their possible remedies, are also described. The small crystalfrontier in micro-crystal crystallography, crystallites in powders and finally down to the proposed single molecule structure determination of X-ray lasers are covered. Overall this interdisciplinary book will interest crystal growers, X-ray and neutron physicists and the biologicalcrystallographers, including graduate students.

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The crystallization of proteins and nucleic acids and/or their complexes has become more highly automated but is still often a trial and error based approach. In parallel, a number of X-ray diffraction based techniques have been developed which explain the physical reasons limiting theresulting crystallographic data and thus show how t...

Naomi E. Chayen is Professor of Biomedical Sciences at Imperial College London, Visiting Professor at Harvard Medical School and the President of the International Organisation for Biological Crystallization (IOBCr). She organises and chairs crystallization sessions at international conferences and courses, and is a Crystallization Co...
Format:HardcoverDimensions:304 pagesPublished:May 1, 2010Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199213259

ISBN - 13:9780199213252

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Table of Contents

I: Introduction and Overview1. IntroductionII: Crystallization2. Crystallization Theory3. Practical Methods of Crystallization4. Screening5. Optimization6. Strategies when high quality crystals cannot be obtained7. Membrane Proteins8. Alternative ApproachesIII: Diffraction9. Experimental Aspects10. Analysis of the Molecular Short-Range Order11. Analysis of the Long-Range Order12. Macromolecular Crystals and Twinning13. Other Macromolecular Crystal Diffraction Disorders14. Degradation and Improvement of Crystal Perfection15. Unusual Diffraction Geometries16. Making the Most of Difficult Crystals17. Protein Powders18. Complementary TechniquesIV: The Future19. The X-ray Laser and the Single Molecule20. Overall Summary and Future ThoughtsGlossary of Abbreviations, Terms, and SymbolsReferencesIndex

Editorial Reviews

"An extremely practical and useful text, especially in these days of high throughput macromolecular crystallography." --Gautam Desiraju, University of Hyderabad