Macromolecular Crystallography: conventional and high-throughput methods

Hardcover | August 23, 2007

EditorMark R. Sanderson, Jane V. Skelly

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Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and forstructure-based function studies to aid the development of effective therapeutic agents and drugs. The successful determination of the complete genome (genetic sequence) of several species (including humans) has recently directed scientific attention towards identifying the structure and function of the complete complement of proteins that make up that species; a new and rapidly growing field ofstudy called 'structural genomics'. There are now several important and well-funded global initiatives in operation to identify all of the proteins of key model species. One of the main requirements for these initiatives is a high-throughput crystallization facility to speed-up the proteinidentification process. The extent to which these technologies have advanced, calls for an updated review of current crystallographic theory and practice. This practical reference book features the latest conventional and high-throughput methods, and includes contributions from a team of internationally recognized leaders and experts. It will be of relevance and use to graduate students, research scientists and professionals currently working in thefield of conventional and high-throughput macromolecular crystallography.

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Macromolecular Crystallography is the study of macromolecules (proteins and nucleic acids) using X-ray crystallographic techniques in order to determine their molecular structure. The knowledge of accurate molecular structures is a pre-requisite for rational drug design, and forstructure-based function studies to aid the development of...

Mark Sanderson is an experienced protein and nucleic-acid crystallographer and co-editor of Methods in Molecular Biology, who has solved several key ab initio structures, notably nucleic-acid proteins. Jane Skelly has widespread experience in the expression and crystallisation of macromolecules. Both editors lecture undergraduates in...

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Format:HardcoverDimensions:280 pages, 9.69 × 7.44 × 0.78 inPublished:August 23, 2007Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0198520972

ISBN - 13:9780198520979

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Table of Contents

1. Skelly, Sohi and Batuwangala: Classical Cloning, Expression and Purification2. Owens, Nettleship, Berrow, Stuart and Stammers: High-Throughput Cloning, Expression and Purification3. Chayen: Automation of Non-Conventional Crystallization Techniques for Screening and Optimisation4. Abdel-Meguid, Jeruzalmi and Sanderson: First Analysis of Macromolecular Crystals5. Sanderson: In-House Macromolecular Data Collection6. Abdel-Meguid: Solving the Phase Problem Using Isomorphous Replacement7. Delarue: Molecular Replacement Techniques for High-Throughput Structure Determination8. Murthy: MAD Phasing9. Weeks and Furey: Application of Direct Methods to Macromolecular Structure Solution10. Abrahams: Phase Refinement through Density Modification11. Morris, Perrakis and Lamzin: Getting a Macromolecular Model: Model Building, Refinement and Validation12. Wasserman, Smith, D'Amico, Koss and Burley: High-Throughput Crystallographic Data Collection at Synchrotons13. Carson: Electron Density Fitting and Structure Validation14. Masquida, Francois, Werner and Westhof: RNA Crystallogenesis15. Sohi and Laponogov: Crystallography in the Study of Protein-DNA Interaction16. Fry, Abrescia and Stuart: Virus Crystallography17. Abdel-Meguid: Macromolecular Crystallography in Drug Design

Editorial Reviews

"It is an excellent, up-to-date review of the methods currently used by x-ray crystallography laboratories around the world." -- The Quarterly Review of Biology, Vol. 83