Malaria Methods and Protocols by Denise L. DoolanMalaria Methods and Protocols by Denise L. Doolan

Malaria Methods and Protocols

EditorDenise L. Doolan

Paperback | November 9, 2010

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Internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria. Areas covered include clinical and laboratory diagnosis and typing, animal models, molecular biology, immunology, cell biology, vaccinology, laboratory models, and field applications. Each readily reproducible protocol has been tested, standardized, and optimized for experimental success, and includes many laboratory notes on troubleshooting, avoiding pitfalls, and interpreting results. Several of the most widely used methods are described here in detail for the first time or have been thoroughly updated since their original publication (e.g., in vitro culture of Plasmodium parasites and in vitro growth inhibition assay).
Title:Malaria Methods and ProtocolsFormat:PaperbackDimensions:646 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372056

ISBN - 13:9781617372056

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Table of Contents

Part I. Assessing Risk of Infection and Severity of DiseaseVector Incrimination and Entomological Inoculation RatesJohn C. BeierEpidemiological Measures of Risk of MalariaJ. Kevin Baird, Michael J. Bangs, Jason D. Maguire, and Mazie J. BarcusPart II. Laboratory ModelsMaintenance of the Plasmodium berghei Life CycleRobert E. Sinden, Geoff A. Butcher, and A. L. BeetsmaMouse Models for Pre-Erythrocytic-Stage MalariaLaurent Rénia, Elodie Belnoue, and Irène LandauMouse Models for Erythrocytic-Stage MalariaLatifu A. Sanni, Luis F. Fonseca, and Jean LanghorneNonhuman Primate Models : I. Nonhuman Primate Host-Parasite CombinationsWilliam E. CollinsNonhuman Primate Models : II. Infection of Saimiri and Aotus Monkeys with Plasmodium vivaxWilliam E. CollinsPart III. Diagnosis and TypingVector AnalysisJohn C. BeierGenotyping of Plasmodium spp.: Nested PCRGeorges SnounouGenotyping of Plasmodium falciparum: PCR-RFLP AnalysisIngrid Felger and Hans-Peter BeckMicrosatellite Analysis in Plasmodium falciparumXin-zhuan Su and Michael T. FerdigQuantitation of Liver-Stage Parasites by Automated TaqMan® Real-Time PCRAdam A. Witney, Robert M. Anthony, and Daniel J. CarucciQuantitation of Liver-Stage Parasites by Competitive RT-PCRKyle C. McKenna and Marcelo R. S. BrionesPart IV. Molecular Biology TechniquesExtraction and Purification of Plasmodium Total RNATill VossExtraction and Purification of Plasmodium Parasite DNAHans-Peter BeckSouthern Blotting of Parasite DNATobias SpielmannSDS-PAGE and Western Blotting of Plasmodium falciparum ProteinsRoland A. CooperNested PCR Analysis of Plasmodium ParasitesGeorges Snounou and Balbir SinghRFLP AnalysisHans-Peter BeckAnalysis of Gene Expression by RT-PCRPeter PreiserIn Situ Detection of RNA in Blood- and Mosquito-Stage Malaria ParasitesJoanne ThompsonPurification of Chromosomes from Plasmodium falciparumDaniel J. Carucci, Paul Horrocks, and Malcolm J. GardnerConstruction of Genomic Libraries from the DNA of Plasmodium SpeciesLeda M. Cummings, Dharmendar Rathore, and Thomas F. McCutchanConstruction of a Gene Library with Mung Bean Nuclease-Treated Genomic DNADharmendar Rathore and Thomas F. McCutchanConstruction of Plasmodium falciparum l cDNA LibrariesDavid A. Fidock, Dharmendar Rathore, and Thomas F. McCutchanProduction of Stage-Specific Plasmodium falciparum cDNA Libraries Using Subtractive HybridizationDavid A. Fidock, Thanh V. Nguyen, Brenda T. Beerntsen, and Anthony A. JamesConstruction and Screening of YAC LibrariesCecilia P. Sanchez, Martin Preuss, and Michael LanzerEpisomal Transformation of Plasmodium bergheiChris J. Janse and Andrew P. WatersGene Targeting in Plasmodium bergheiVandana Thathy and Robert MénardPart V. Immunological TechniquesPeptide Vaccination, Valentin MeraldiJackeline F. Romero, and Giampietro CorradinDNA VaccinationRichard C. Hedstrom and Denise L. DoolanAssessing Antigen-Specific CD8+ and CD4+ T-Cell Responses in Mice After Immunization with Recombinant VirusesMoriya TsujiAssessing CD4+ Helper T-Lymphocyte Responses by LymphoproliferationIsabella A. Quakyi and Jeffrey D. AhlersLimiting Dilution Analysis of Antigen-Specific CD4+ T-Cell Responses in MiceElsa Seixas, Jean Langhorne, and Stuart QuinCell Trafficking: Malaria Blood-Stage Parasite-Specific CD4+ T Cells After Adoptive Transfer into MiceChakrit Hirunpetcharat and Michael F. GoodAssessing Antigen-Specific Proliferation and Cytokine Responses Using Flow CytometryCatherine E. M. Allsopp and Jean LanghorneCytokine Analysis by Intracellular StainingAftab A. Ansari and Ann E. MayneAssessment of Antigen-Specific CTL- and CD8+-Dependent IFN-g Responses in MiceKatrin Peter, Régine Audran, Anilza Bonelo, Giampietro Corradin, and José Alejandro LópezAssessing Antigen-Specific CD8+ CTL Responses in HumansDenise L. DoolanHuman Antibody Subclass ELISAPierre Druilhe and Hasnaa Bouharoun-TayounSystemic Nitric Oxide Production in Human Malaria: I. Analysis of NO Metabolites in Biological FluidsNicholas M. Anstey, Craig S. Boutlis, and Jocelyn R. SaundersSystemic Nitric Oxide Production in Human Malaria: II. Analysis of Mononuclear Cell Nitric Oxide Synthase Type 2 Antigen ExpressionJocelyn R. Saunders, Mary A. Misukonis, J. Brice Weinberg, and Nicholas M. AnsteyPart VI. Cell Biology TechniquesIn Vitro Culture of Plasmodium ParasitesJames B. JensenAutomated Synchronization of Plasmodium falciparum Parasites by Culture in a Temperature-Cycling IncubatorJ. David Haynes and J. Kathleen MochHepatic Portal Branch InoculationJohn B. Sacci, Jr.Hepatocyte Perfusion, Isolation, and CultureJohn B. Sacci, Jr.Inhibition of Sporozoite Invasion: The Double-Staining AssayLaurent Rénia, Ana Margarida Vigário, and Elodie BelnoueInhibition of Liver-Stage Development AssayJohn B. Sacci, Jr.T-Cell Mediated Inhibition of Liver-Stage Development AssayLaurent Rénia, Elodie Belnoue, and Ana Margarida VigárioAntibody-Dependent Cellular Inhibition AssayPierre Druilhe and Hasnaa Bouharoun-TayounErythrocytic Malaria Growth or Invasion Inhibition Assays with Emphasis on Suspension Culture GIAJ. David Haynes, J. Kathleen Moch, and Douglas S. SmootAnalysis of CSA-Binding Parasites and Antiadhesion AntibodiesMichal Fried and Patrick E. DuffyAnalysis of the Adhesive Properties of Plasmodium falciparum-Infected Red Blood Cells Under Conditions of FlowBrian M. Cooke, Ross L. Coppel, and Gerard B. NashPreparation of Adhesive Targets for Flow-Based Cytoadhesion AssaysBrian M. Cooke, Ross L. Coppel, and Gerard B. NashTriton X-114 Phase Partitioning for Antigen CharacterizationLina Wang and Ross L. CoppelImmunoprecipitation for Antigen LocalizationJohn G. T. Menting and Ross L. CoppelPart VII. Testing Interventions For Malaria ControlField TrialsPedro L. Alonso and John J. AponteIndex

Editorial Reviews

"In this book, internationally respected scientists and clinicians describe in step-by-step detail their most useful conventional and cutting-edge techniques for the study of malaria." - Clinical Laboratory International"This is an outstanding collection of protocols written by over eighty experts in the field. The geographical spread of these contributors is impressive, encompassing writers from thirteen countries on six continents. There is good stylistic continuity throughout the book, despite the diversity of the authors...there is admirable cross-referencing between chapters." -Molecular Biotechnology