Malaria: Methods and Protocols by Robert MénardMalaria: Methods and Protocols by Robert Ménard

Malaria: Methods and Protocols

EditorRobert Ménard

Hardcover | September 19, 2012

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Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical,  Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory  researchers.
Title:Malaria: Methods and ProtocolsFormat:HardcoverDimensions:540 pages, 10 × 7.01 × 0.01 inPublished:September 19, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627030255

ISBN - 13:9781627030250

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Table of Contents

Part I. Parasite Culturing 1. In vitro Culturing P. falciparum Erythrocytic StagesAlexander G. Maier and Melanie Rug 2. Production of Plasmodium falciparum Gametocytes in vitroKathryn Shaw Saliba and Marcelo Jacobs-Lorena 3. In vitro Differentiation of Plasmodium falciparum Gametocytes into OokinetesAnil K. Ghosh and Marcelo Jacobs-Lorena 4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken 5. Laboratory Maintenance of Rodent Malaria ParasitesChandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden Part II. Genome Manipulation 6. Transfection of Plasmodium falciparumMelanie Rug and Alexander G. Maier 7. Transfection of Rodent Malaria ParasitesNisha Philip, Rachael Orr, and Andrew P. Waters 8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNAClaudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker 9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse 10. Transient Transfection of Plasmodium vivax Blood-stage ParasitesCecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer 11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  3. In vitro Differentiation of Plasmodium falciparum Gametocytes into OokinetesAnil K. Ghosh and Marcelo Jacobs-Lorena 4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken 5. Laboratory Maintenance of Rodent Malaria ParasitesChandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden Part II. Genome Manipulation 6. Transfection of Plasmodium falciparumMelanie Rug and Alexander G. Maier 7. Transfection of Rodent Malaria ParasitesNisha Philip, Rachael Orr, and Andrew P. Waters 8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNAClaudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker 9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse 10. Transient Transfection of Plasmodium vivax Blood-stage ParasitesCecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer 11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  3. In vitro Differentiation of Plasmodium falciparum Gametocytes into OokinetesAnil K. Ghosh and Marcelo Jacobs-Lorena 4. Ex vivo culture of Plasmodium Vivax and Plasmodium Cynomolgi and in vitro Culture of Plasmodium Knowlesi Blood Stages Anne-Marie Zeeman, Annemarie Voorberg-van der Wel, and Clemens H. M. Kocken 5. Laboratory Maintenance of Rodent Malaria ParasitesChandra Ramakrishnan, Michael J. Delves, Kalpana Lal, Andrew M. Blagborough, Geoffrey Butcher, Kenneth W. Baker, and Robert E. Sinden Part II. Genome Manipulation 6. Transfection of Plasmodium falciparumMelanie Rug and Alexander G. Maier 7. Transfection of Rodent Malaria ParasitesNisha Philip, Rachael Orr, and Andrew P. Waters 8. Recombination-mediated Genetic Engineering of Plasmodium berghei DNAClaudia Pfander, Burcu Anar, Mathieu Brochet, Julian C. Rayner, and Oliver Billker 9. Standardization in Generating and Reporting Genetically Modified Rodent Malaria Parasites: The RMgmDB Database Shahid M. Khan, Hans Kroeze, Blandine Franke-Fayard, and Chris J. Janse 10. Transient Transfection of Plasmodium vivax Blood-stage ParasitesCecilia P. Sanchez, Judith Pfahler, Hernando A del Portillo, and Michael Lanzer 11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  11. Silencing of Genes and Alleles by RNAi in Anopheles gambiaeMarina Lamacchia, John R. Clayton, Rui Wang-Sattler, Lars M. Steinmetz, Elena A. Levashina, and Stéphanie A. Blandin Part III. 'OMIC' Techniques 12. Analysis of Variant Gene Family Expression by Quantitative PCRSohini Sanyal, Thomas J. Templeton, and Cristina K. Moreira 13. DNA Microarray-based Genome-wide Analyses of Plasmodium ParasitesZbynek Bozdech, Sachel Mok, and Archna P. Gupta 14. Whole-genome Analysis of Plasmodium spp. Utilizing a New Agilent Technologies DNA Microarray PlatformHeather J. Painter, Lindsey M. Altenhofen, Björn F.C. Kafsack and Manuel Llinás 15. Transcriptome Analysis using RNA-seqWieteke A.M. Hoeijmakers, Richárd Bártfai, and Hendrik G. Stunnenberg  16. Experimental Tools for the Study of Protein Phosphorylation in PlasmodiumDominique Dorin-Semblat, Andrew R. Bottrill, Lev Solyakov, Andrew Tobin, and Christian Doerig 17. Extraction of Hydrophilic Metabolites from Plasmodium falciparum-infected Erythrocytes for Metabolomic AnalysisKellen L. Olszewski and Manuel Llinás Part IV. Red Blood Cell Stages 18. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparumDavid T. Riglar and Jake Baum 19. Flow Cytometry-based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum MerozoitesShailja Singh and Chetan E. Chitnis 20. Microsphiltration: a Microsphere Matrix to Explore Erythrocyte DeformabilityCatherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon, Peter H. David, and Pierre Buffet 21. Atomic Force Microscopy of Plasmodium-infected Red Blood Cells for Detecting and Localizing Single Molecular Recognition EventsAng Li, Laurent Rénia, Chwee Teck Lim, and Bruce Russell 22. Expressing Full-length Functional PfEMP1 Proteins in the HEK293 Expression System Anand Srivastava, Yves Durocher, and Benoît Gamain 23. Genome-wide Chromatin Immunoprecipitation-sequencing in PlasmodiumJose-Juan Lopez-Rubio, T. Nicolai Siegel, and Artur Scherf 24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  24. In situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparumLiliana Mancio-Silva and Artur Scherf 25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  25. Bioluminescence Imaging of P. berghei Schizont Sequestration in RodentsJoanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, and Blandine Franke-Fayard Part V. Pre-Erythrocytic Stages26. Scoring Sporozoite MotilityAllison F. Carey, Robert Ménard, and Daniel Y. Bargieri 27. Quantification of Sporozoite Invasion, Migration and Development by Microscopy and Flow Cytometry Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, and Maria M. Mota 28. Imaging Sporozoite Cell Traversal in the Liver of Mice Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, and Rogerio Amino 29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  29. Analysis of Liver Stage Development in, and Merozoite Release from HepatocytesRebecca R. Stanway, Jacqueline Schmuckli-Maurer, and Volker T. Heussler 30. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence ImagingTakeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, and Shahid M. Khan Part VI. Immunity to Plasmodium31. Activation of Human NK Cells by Plasmodium-infected Red Blood CellsAmir Horowitz and Eleanor M. Riley 32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti  32. Chimeric Parasites as Tools to Study Plasmodium  Immunology and Assess Malaria VaccinesIan Cockburn 33. Development and Use of TCR Transgenic Mice for Malaria Immunology ResearchYun-Chi Chen and Fidel Zavala 34. Tracking the Total CD8 T Cell Response Following Whole Plasmodium Vaccination Katherine L. Doll, Noah S. Butler, and John T. Harty         Part VII. Controlling Plasmodium Infection 35. Screening Inhibitors of P. berghei Blood Stages using Bioluminescent Reporter ParasitesJing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse, and Blandine Franke-Fayard 36. Screening and Evaluation of Inhibitors of Plasmodium falciparum Merozoite Egress and Invasion using CytometryAnthony Bouillon, Olivier Gorgette, Odile Mercereau-Puijalon, and Jean-Christophe Barale 37. Induction of anti-Plasmodium Immunity Following Sub-patent Infection with Live Erythrocytic Stages and Drug CureDanielle I. Stanisic and Michael F. Good 38. Vaccination using Radiation-or Genetically-Attenuated Live SporozoitesAshley M Vaughan and Stefan H I Kappe39. Vaccination using Normal Live Sporozoites Under Drug TreatmentLaurent Rénia, Anne Charlotte Grüner, Marjorie Mauduit, and Georges Snounou  40. Assessing Transmission Blockade in Plasmodium spp. Andrew M. Blagborough, Michael J. Delves, Chandra Ramakrishnan, Kalpana Lal, Geoffrey Butcher, and Robert E. Sinden 41. Mosquito Transgenic Technologies to Reduce Plasmodium TransmissionSilke Fuchs, Tony Nolan, and Andrea Crisanti