Mammalian Chromosome Engineering: Methods and Protocols by Gyula HadlaczkyMammalian Chromosome Engineering: Methods and Protocols by Gyula Hadlaczky

Mammalian Chromosome Engineering: Methods and Protocols

EditorGyula Hadlaczky

Hardcover | April 6, 2011

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The rapid progression of genetics and molecular biology has turned chromosomal engineering from science fiction to reality, with the successful production of transgenic animals with engineered chromosomes and chromosomes developed for pharmaceutical protein production which are now ready for the medical industry.  Mammalian Chromosome Engineering: Methods and Protocols provides the reader with up-to date information on this rapidly evolving field and strives to take the reader into the exciting realm of chromosomal engineering from the basic principles to the practical applications of these new technologies. The five overview and ten protocol chapters cover the engineering of chromosomes with extrachromosomal vectors and transposon systems, the manipulation of naturally occurred minichromosomes, the generation and engineering of synthetic artificial chromosomes, and the induced de novo platform artificial chromosome system.  Written in the highly successful Methods in Molecular BiologyT series format, protocols chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Mammalian Chromosome Engineering: Methods and Protocols serves as a bench-side resource for current protocols and aims to help scientists to explore the many prospects for future research and vital applications.
Title:Mammalian Chromosome Engineering: Methods and ProtocolsFormat:HardcoverDimensions:266 pagesPublished:April 6, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617790982

ISBN - 13:9781617790980

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Table of Contents

1. Developing Extrachromosomal Gene Expression Vector Technologies: An Overview            Richard Wade-Martins 2. High Capacity Extrachromosomal Gene Expression Vectors            Olivia Hibbitt and Richard Wade-Martins 3. Naturally Occurring Minichromosome Platforms in Chromosome Engineering: An Overview            Elena Raimondi 4. Chromosome Transfer Via Cell Fusion            Marianna Paulis 5. Insertional Engineering of Chromosomes with Sleeping Beauty Transposition: An Overview            Ivana Grabundzija, Zsuzsanna Izsvák, and Zoltán Ivics 6. Rodent Transgenesis Mediated by a Novel Hyperactive Sleeping Beauty Transposon System            Lajos Mátés 7. Construction and Use of a Bottom-Up HAC Vector for Transgene Expression            Masashi Ikeno and Nobutaka Suzuki 8. De novo Generation of Satellite DNA-Based Artificial Chromosomes by Induced Large-Scale Amplification            Erika Csonka 9. Downstream Bioengineering of ACE Chromosomes for Incorporation of Site-Specific Recombination Cassettes            Amy L. Greene and Edward L. Perkins 10. Chromosome Engineering with Lambda-Integrase Mediated Recombination System: The ACE System            Tünde Praznovszky 11. Dendrimer Mediated Transfer of Engineered Chromosomes            Robert L. Katona 12. Engineered Chromosomes in Transgenics            Peter Blazso, Ildiko Sinko, and Robert L. Katona 13. Transfer of Stem Cells Carrying Engineered Chromosomes with XY Clone Laser System            Ildiko Sinko and Robert L. Katona 14. Mammalian Artificial Chromosomes and Clinical Applications for Genetic Modification of Stem Cells: An Overview            Robert L. Katona, Sandra L. Vanderbyl, and Carl F.Perez 15. Engineered Mammalian Chromosomes in Cellular Protein Production: Future Prospects            Malcolm L. Kennard