Manual of Techniques in Invertebrate Pathology by Lawrence A. LaceyManual of Techniques in Invertebrate Pathology by Lawrence A. Lacey

Manual of Techniques in Invertebrate Pathology

byLawrence A. Lacey, Lawrence A. Lacey

Other | April 25, 2012

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Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management.
Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy.
Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful.

  • Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens
  • New edition fully updated to address changes in the taxonomy of the vast majority of taxa
  • Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques
  • Provides extensive supplemental literature and recipes for media, fixatives and stains
Dr. Lacey earned his PhD in 1978 and has been internationally active working in medical entomology, insect pathology, and microbial control of economically and medically important insects. He conducted research on the biology and microbial control of dozens of insects in the US, Amazon Basin, West Africa in the Azores, throughout Europ...
Title:Manual of Techniques in Invertebrate PathologyFormat:OtherDimensions:504 pages, 1 × 1 × 1 inPublished:April 25, 2012Publisher:Elsevier ScienceLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0123869005

ISBN - 13:9780123869005


Table of Contents

Manual of Techniques in Invertebrate Pathology, 2ndedition

Lawrence A. Lacey, editor

1. Initial Handling and Diagnosis of Diseased Invertebrates

Lawrence A. Lacey and Leellen Solter

2. Basic Techniques in Insect Virology

Karolin E. Eberle, Jörg T. Wennmann, Regina G. Kleespiesand Johannes A. Jehle

3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli.

Tanja W. Fisher and Steven F. Garczynski

4. Bioassay of Bacterial Entomopathogens Against Insect Larvae

Maureen O'Callaghan, Travis R. Glare and Lawrence A. Lacey

5. Bacteria for use Against Soil-Inhabitating Insects

Albrecht M. Koppenhöfer,Trevor A. Jackson, and Michael G. Klein

6. Identification of Entomopathogenic Fungi

Richard A. Humber

7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales

G. Douglas Inglis, Juerg Enkerli,and Mark S. Goettel

8. Mass Production of Entomopathogenic Hypocreales

Stefan T. Jaronski and Mark A. Jackson

9. Methods for the Study of Entomophthorales

Ann E. Hajek, Bernard Papierok, and Jorgen Eilenberg

10. Preservation of Entomopathogenic Fungal Cultures

Richard A. Humber

11. Research Methods for Entomopathogenic Microsporidia and Other Protists

Leellen Solter, James Becnel, and Jiri Vávra

12. Nematode Parasites, Pathogens and Associates of Insects and Invertebrates of Economic Importance

S. Patricia Stock and Heidi Goodrich-Blair

13. Pathogens and Parasites of Terrestrial Molluscs

Michael J. Wilson

14. Testing the Pathogenecity and Infectivity of Entomopathogens to Mammals

Joel P. Siegel

15. Complementary Techniques: Preparations of Entomopathogens and Diseased Specimens for More Detailed Study Using Microscopy

James J. Becnel