Marijuana and Cannabinoid Research: Methods and Protocols by Emmanuel S. OnaiviMarijuana and Cannabinoid Research: Methods and Protocols by Emmanuel S. Onaivi

Marijuana and Cannabinoid Research: Methods and Protocols

EditorEmmanuel S. Onaivi

Paperback | November 10, 2010

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A cutting-edge collection of readily reproducible in vitro and in vivo methods to elucidate the mechanisms associated with cannabinoid function in health and disease. The techniques can be used in studies across the board from genes to behavior. The molecular neurobiological methods are invaluable in analyzing the structure, the polymorphisms, and the molecular expression of the cannabinoid receptors (CBrs), as well as their association with polysubstance abuse. There are also methods for localizing cannabinoid receptors in different systems, visualizing cannabinoid effects using brain slice imaging and elctrophysiological approaches, and designing and synthesizing cannabinoids and endocannabinoids. The protocols follow the successful Methods in Molecular MedicineT series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Title:Marijuana and Cannabinoid Research: Methods and ProtocolsFormat:PaperbackDimensions:322 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375136

ISBN - 13:9781617375132

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Table of Contents

Molecular Neurobiological Methods in Marijuana-Cannabinoid ResearchGeorge R. Uhl, Hiroki Ishiguro, Emmanuel S. Onaivi, Zhicheng Lin, B. Emmanuel Akinshola, Bruce Hope, Claire M. Leonard, and Qing-Rong LiuExperimental Methods to Study the Role of the Peripheral Cannabinoid Receptor in Immune FunctionNancy E. Buckley, Diep Burbridge, Manop Buranapramest, Tanya Ferguson, and Renee Y. PaauLocalization of Cannabinoid Receptors Using Immunoperoxidase MethodsGuy A. CabralLocalization of Cannabinoid CB1 Receptor mRNA Using Ribonucleotide Probes: Methods for Double- and Single-Label In Situ HybridizationAndrea G. HohmannMorphometric Study on Cytoskeletal Components of Neuronal and Astroglial Cells After a Chronic CB1 Agonist TreatmentPatricia Tagliaferro, Alberto J. Ramos, Emmanuel S. Onaivi, Sergio G. Evrard, Maite Duhalde Vega, and Alicia BruscoVisualizing Cannabinoid Effects Using Brain Slice Imaging and Electrophysiological ApproachesAlexander F. Hoffman and Carl R. LupicaMethods for the Synthesis of Cannabinergic LigandsGanesh A. Thakur, Spyros P. Nikas, Richard I. Duclos, Jr., and Alexandros MakriyannisCannabinoid Receptor Binding to Membrane Homogenates and Cannabinoid-Stimulated [35S]GTPgS Binding to Membrane Homogenates or Intact Cultured CellsChris BreivogelMethods to Assay Anandamide Hydrolysis and Transport in SynaptosomesFilomena Fezza, Natalia Battista, Monica Bari, and Mauro MaccarroneMethods Evaluating Cannabinoid and Endocannabinoid Effects on Gastrointestinal FunctionsNissar A. DarmaniThe Bioassay of Cannabinoids Using the Mouse Isolated Vas DeferensAd`ele Thomas and Roger G. PertweeAssessment of Cannabis Craving Using the Marijuana Craving QuestionnaireStephen J. Heishman and Edward G. SingletonMethods for Studying Acute and Chronic Effects of Marijuana on Human Associative Processes and MemoryRobert I. BlockMethods for Clinical Research Involving Cannabis AdministrationDavid A. Gorelick and Stephen J. HeishmanNeurological Assessments of Marijuana UsersJean Lud Cadet, Karen Bolla, and Ronald I. HerningBehavioral Methods in Cannabinoid ResearchEster Fride, Alex Perchuk, F. Scott Hall, George R. Uhl, and Emmanuel S. OnaiviMethods to Study the Behavioral Effects and Expression of CB2 Cannabinoid Receptors and Its Gene Transcripts in Chronic Mild Stress Model of DepressionEmmanuel S. Onaivi, Hiroki Ishiguro, Patel Sejal, Paul A. Meozzi, Lester Myers, Patricia Tagliaferro, Bruce Hope, Claire M. Leonard, George R. Uhl, Alicia Brusco, and Eileen GardnerIndex

Editorial Reviews

"...will be a great asset internationally to emerging researchers embarking on investigations of cannabinoid seeking further understanding in their specific field, by expanding upon their exisiting methodologies to incorporate multi-disciplinary approaches." -Addiction