Mass Spectrometry: Techniques For Structural Characterization Of Glycans

Hardcover | May 16, 2016

byMichael A. Madson

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Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules. As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study. Authored by an analytical chemist with more than 30 years of experience in research and industry Serves as a quick reference in mass spectral analysis and carbohydrates Includes more than 60 figures to aid in the retention of key concepts

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Mass Spectrometry: Techniques for the Structural Characterization of Glycans presents new methods for conducting detailed carbohydrate qualitative analysis arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules. As the...

Mike Madson is CEO and Senior Research Scientist of BioLogistics, LLC, Iowa, USA

other books by Michael A. Madson

Format:HardcoverDimensions:86 pages, 9.41 × 7.24 × 0.98 inPublished:May 16, 2016Publisher:Elsevier Science & TechLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128041293

ISBN - 13:9780128041291

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Table of Contents

" Introduction
" Monosaccharide Composition Analysis
" Elemental Analysis of MS Ions
" Derivatization Techniques
" Hydride Addition to Sulfate and phosphate- Chemistry
" Instrumentation
MALDI-TOF MS
API 2000 MS
ICP ESI TOF MS
" Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters
" Ester Substitution MS Method
The Protocol for the Hydride Addition to the ethanol extract of banana fruit
Example of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan
" Glycan Isolation Methods
" Other Glycan isolation Methods
" Monosaccharide Analysis
Acid Hydrolysis
" Mass Spectral Carbohydrate Structure Determination Method
MS
MS/MS (MS2)
MSn
Proposing Initial Structure
Refining Methods for Determining Exact Mass Structure
MS Instrumentation Requirements for Discovering Whole Molecule Ions
Derivatization Method and Sulfate/Phosphate Discernment Method
" References