Mass Spectrometry: Techniques For Structural Characterization Of Glycans by Michael A. MadsonMass Spectrometry: Techniques For Structural Characterization Of Glycans by Michael A. Madson

Mass Spectrometry: Techniques For Structural Characterization Of Glycans

byMichael A. Madson

Hardcover | May 16, 2016

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Mass Spectrometry: Techniques for the Structural Characterization of Glycanspresents new methods for conducting detailed carbohydrate qualitative analysis arming analytical chemists, pharmaceutical scientists, and food scientists with a quick reference that will allow them to determine the structures of carbohydrates molecules.

As there is a need in the scientific community for content specific to structural determination and analysis of new glycoprotein drug, and because structure-activity analysis requires a structural determination of the N- and O-linked oligosaccharides linked to glycol-proteins, this book provides the relevant research that are necessary for advances and new outcomes in this area of study.

  • Authored by an analytical chemist with more than 30 years of experience in research and industry
  • Serves as a quick reference in mass spectral analysis and carbohydrates
  • Includes more than 60 figures to aid in the retention of key concepts
Mike Madson is CEO and Senior Research Scientist of BioLogistics, LLC, Iowa, USA
Title:Mass Spectrometry: Techniques For Structural Characterization Of GlycansFormat:HardcoverDimensions:86 pages, 9.41 × 7.24 × 0.98 inPublished:May 16, 2016Publisher:Elsevier ScienceLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128041293

ISBN - 13:9780128041291

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Table of Contents

" Introduction
" Monosaccharide Composition Analysis
" Elemental Analysis of MS Ions
" Derivatization Techniques
" Hydride Addition to Sulfate and phosphate- Chemistry
" Instrumentation
API 2000 MS
" Hydride Insertion Addition Reaction for the Derivatization of Phosphorylated or Sulfated Glycans and the Discernment of Phosphate versus Sulfate Esters
" Ester Substitution MS Method
The Protocol for the Hydride Addition to the ethanol extract of banana fruit
Example of MS2on Hydride added to the ethanol Extract of Banana Fruit Glycan
" Glycan Isolation Methods
" Other Glycan isolation Methods
" Monosaccharide Analysis
Acid Hydrolysis
" Mass Spectral Carbohydrate Structure Determination Method
Proposing Initial Structure
Refining Methods for Determining Exact Mass Structure
MS Instrumentation Requirements for Discovering Whole Molecule Ions
Derivatization Method and Sulfate/Phosphate Discernment Method
" References