Measurement And Analysis Of Kinetic Isotope Effects by Michael E. Harris

Measurement And Analysis Of Kinetic Isotope Effects

byMichael E. HarrisEditorVernon Anderson

Hardcover | September 10, 2017

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Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 596,the latest release in theMethods in Enzymologyseries, continues the legacy of this premier serial with quality chapters authored by leaders in the field.

The best, and arguably the only, way to interrogate the transition states of enzyme transition states is kinetic isotope effect analyses. However, technical challenges have limited the application of this powerful approach to a relatively small number of systems. This volume provides the reader who is interested in applying or understanding this research with a survey the major methods currently used for measuring isotope effects on solution and enzyme reactions.



  • Written by pioneers of modern isotope effect research
  • Is the only collection of modern kinetic isotope effect methods currently available

About The Author

Dr. Michael E. Harris received his BS in Chemistry from Florida State University in 1986. While an undergraduate at FSU he worked in the lab of Dr. William F. Marzluff on the cell cycle regulation of histone mRNA stability. In 1992 he began graduate school at the University of Alabama at Birmingham in the laboratory of Dr. Stephen L. H...
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Details & Specs

Title:Measurement And Analysis Of Kinetic Isotope EffectsFormat:HardcoverDimensions:412 pages, 9.41 × 7.24 × 0.98 inPublished:September 10, 2017Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128122730

ISBN - 13:9780128122730

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Table of Contents

  1. Measurement of enzyme binding isotope effects
    Vern Schramm
  2. Chemical ligation and isotope labeling to locate dynamic effects
    Rudolf Alleman
  3. Measurement of heavy enzyme isotope effects
    Amnon Kohen
  4. Extracting kinetic isotope effects from a global analysis of reaction progress curves
    Sam Hay
  5. KIE of metabolic flux and enzymes
    Guillaume Tcherkez
  6. Solvent and Primary KIE on Flavin Enzymes
    Paul Fitzpatrick
  7. The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD
    John Richard
  8. 18-O isotope effects on oxygenases
    Judith Klinman
  9. Chlorine isotope effects on dehalogenases
    Piotr Paneth
  10. Chlorine isotope effects (w/Paneth)
    Agnieszka Dybala-Defratyka
  11. Kinetic Deuterium Isotope Effects in Cytochrome P450 Reactions
    F. Peter Guengerich
  12. Use of Isotopes and Isotope Effects for Investigations of Diiron Oxygenase Mechanisms
    John Lipscomb
  13. Characterization of substrate, co-substrate, and product isotope effects associated with enzymatic oxygenations using gas- and liquid chromatography/isotope ratio mass spectrometry
    Thomas Hofstetter
  14. NMR titrations to determine equilibrium isotope effects
    Charles Perrin
  15. NMR to characterize the nonstatistical distribution of stable isotopes in metabolism
    Richard Robins
  16. Applications of isotope ratio mass spectrometry in sports drug testing: accounting for isotope fractionation in analysis of biological samples
    Thomas Piper
  17. Whole molecule mass spectrometry to measure KIEs on RNA 2 O-transphosphorylation reactions
    Mike Harris
  18. Isotope Effects on the Concentration of Steady State Intermediates
    Hung-Wen Liu and Mark Ruszczycky
  19. Headspace analysis of CO2 to measure carbon kinetic isotope effects in decarboxylation reactions
    Ron Kluger
  20. 32P/33P remote label methods to measure RNA kinetic isotope effects
    Joseph Piccirilli
  21. Isotope effects and MS analysis of pseudouridine synthase enzymes
    Eugene Mueller
  22. Nucleophile isotope effects on glycosyl transfer reactions
    Andrew James Bennet