Measurement And Analysis Of Kinetic Isotope Effects

Hardcover | September 10, 2017

byMichael E. HarrisEditorVernon Anderson

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Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 595, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Written by leading authorities Covers the experimental analysis of enzyme mechanism using isotope effects

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Experimental Analysis of Enzyme Mechanism Using Isotope Effects, Volume 595, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Written by leading authorities Covers the experimental analysis of enzyme mechanism using isotope effect...

Prof. Michael E. Harris works at the Department of Biochemistry, Case Western Reserve University, USAVernon Anderson, is a program director in the Division of Pharmacology, Physiology, and Biological Chemistry, National Institute of General Medical Sciences, Bethesda, USA

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Format:HardcoverDimensions:412 pages, 9.41 × 7.24 × 0.98 inPublished:September 10, 2017Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128122730

ISBN - 13:9780128122730

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Table of Contents

  1. Measurement of enzyme binding isotope effects
    Vern Schramm
  2. Chemical ligation and isotope labeling to locate dynamic effects
    Rudolf Alleman
  3. Measurement of heavy enzyme isotope effects
    Amnon Kohen
  4. Extracting kinetic isotope effects from a global analysis of reaction progress curves
    Sam Hay
  5. KIE of metabolic flux and enzymes.
    Guillaume Tcherkez
  6. Solvent and Primary KIE on Flavin Enzymes.
    Paul Fitzpatrick
  7. The Rapid Determination of Primary Deuterium Isotope Effects on Enzyme-Catalyzed Proton Transfer at Carbon in 50/50 HOH/DOD
    John Richard
  8. 18-O isotope effects on oxygenases
    Judith Klinman
  9. Chlorine isotope effects on dehalogenases
    Piotr Paneth
  10. Chlorine isotope effects (w/Paneth)
    Agnieszka Dybala-Defratyka
  11. Kinetic Deuterium Isotope Effects in Cytochrome P450 Reactions
    F. Peter Guengerich
  12. Use of Isotopes and Isotope Effects for Investigations of Diiron Oxygenase Mechanisms
    John Lipscomb
  13. Characterization of substrate, co-substrate, and product isotope effects associated with enzymatic oxygenations using gas- and liquid chromatography/isotope ratio mass spectrometry
    Thomas Hofstetter
  14. NMR titrations to determine equilibrium isotope effects
    Charles Perrin
  15. NMR to characterize the nonstatistical distribution of stable isotopes in metabolism
    Richard Robins
  16. Applications of isotope ratio mass spectrometry in sports drug testing: accounting for isotope fractionation in analysis of biological samples
    Thomas Piper
  17. Whole molecule mass spectrometry to measure KIEs on RNA 2 O-transphosphorylation reactions
    Mike Harris
  18. Isotope Effects on the Concentration of Steady State Intermediates
    Hung-Wen Liu and Mark Ruszczycky
  19. Headspace analysis of CO2 to measure carbon kinetic isotope effects in decarboxylation reactions
    Ron Kluger
  20. 32P/33P remote label methods to measure RNA kinetic isotope effects
    Joseph Piccirilli
  21. Isotope effects and MS analysis of pseudouridine synthase enzymes
    Eugene Mueller
  22. Nucleophile isotope effects on glycosyl transfer reactions
    Andrew James Bennet