Membrane Transport: A Practical Approach

Paperback | June 15, 2000

EditorStephen A. Baldwin

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Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, totechniques for investigation of their structures. As such, it not only proves the necessary technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with recent developments in these areas. While some of the approaches described requiresophisticated equipment (e.g. a stopped-flow fluorimeter), most of the protocols can be implemented in any well- found laboratory. Preparation of this volume comes at a time when a result of genome sequencing our knowledge of membrane transporter sequences is far outstripping our understanding oftheir molecular mechanisms. Our hope is that this book will help future researchers to redress this imbalance.

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Membrane Transport is targeted towards researchers with an interest in the mechanism of solute transport across biological membranes. Its scope is broad, ranging from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, totechniques for investigation of their struc...

Stephen A. Baldwin is at University of Leeds.

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Format:PaperbackPublished:June 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637040

ISBN - 13:9780199637041

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Table of Contents

Assay of membrane transport in cells and membrane vesiclesReconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulumThe Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteinsExpression of foreign transport proteins in yeastBaculovirus-mediated overexpression of transport proteinsThe amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteinsSpectroscopic and kinetic approaches for probing the mechanisms of solute transportersDetection and analysis of glucose transporters using photolabelling techniquesComputer prediction of transporter topology and structureTwo-dimensional crystallization of membrane proteins

Editorial Reviews

'Stephen A.Baldwin has performed a service to the scientific community in compelling these technical approaches to membrane transport studies' Journal Cell Science