Metastasis Research Protocols by Susan A. BrooksMetastasis Research Protocols by Susan A. Brooks

Metastasis Research Protocols

EditorSusan A. Brooks

Paperback | November 9, 2010

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Metastasis Research Protocols describes in detail all the methods needed to investigate why and how metastasis occurs. Volume I presents a comprehensive collection of established and leading-edge techniques for analyzing the expression of key molecules and for examining their production at the genetic level. The work focuses on the analysis and mapping of molecules produced by cells and tissues, and on the molecular biology underlying their expression. The traditional methods range from the histopathological and the immunocytochemical to SDS-PAGE, Western blotting, and enzyme zymography. Newer and more specialized techniques for analyzing the genetic aspects of metastasis include in situ hybridization to localize mRNAs, FISH, CGH, methylation analysis of CpG islands, RT-PCR, and differential display. Volume II presents a comprehensive collection of established and leading-edge techniques for examining metastatic behavior in vitro and whole animal models. Methods are provided for the separation of cell lines with high and low metastatic potential, along with assay systems to test defined aspects of the metastatic cascade. These systems include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The detailed descriptions of animal models of metastasis cover the use of immunodeficient animals, syngenic and transgenic models, and orthotopic models of metastasis.
Title:Metastasis Research ProtocolsFormat:PaperbackDimensions:344 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617370967

ISBN - 13:9781617370960

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Table of Contents

Part I. Detection Methods for Cellular Markers of Metastatic PotentialHistopathological Assessment of MetastasisDerek E. Roskell and Ian D. BuleyBasic Immunocytochemistry for Light MicroscopySusan A. BrooksMultiple Labeling Techniques for Fluorescence MicroscopyAmanda J. Atherton and Catherine ClarkeLectin Histochemistry to Detect Altered Glycosylation in Cells and TissuesSusan A. Brooks and Debbie M. S. HallImmunocytochemical Detection and Characterization of Individual Micrometastatic Tumor CellsStephan Braun and Klaus PantelUnderstanding Metastasis Through Integrin ExpressionLawrence John and Massimo PignatelliSurrogate Markers of Angiogenesis and MetastasisGirolamo Ranieri and Giampietro GaspariniMeasurement of Microvessel Density in Primary TumorsHelen E. Turner and Adrian L. HarrisAssessment of Cellular Proliferation by Calculation of Mitotic Index and by ImmunohistochemistryCheryl E. GillettFlow Cytometric Measurement of Cell ProliferationRichard S. CamplejohnSDS-PAGE and Western Blotting TechniquesChristine Blancher and Adam JonesAssessment of Gelatinases (MMP-2 and MMP-9) by Gelatin ZymographyMarta Toth and Rafael FridmanPart II. Genetic Aspects of MetastasisIn Situ Hybridization to Localize mRNAsRichard PoulsomFluorescence In Situ HybridizationHakan Goker and Janet ShipleyComparative Genomic HybridizationRebecca RoylanceRNA Purification and Quantification MethodsAdam Jones, Chisato Fujiyama, and Kenneth SmithNorthern and Southern Blotting and the Polymerase Chain Reaction to Detect Gene ExpressionThelma Tennant, Marina Chekmareva, and Carrie W. Rinker-SchaefferMethylation Analysis of CpG IslandsRichard Lilischkis, Hermann Kneitz, and Hans KreipeDetection of Metastatic Tumor Cells in Blood by Reverse Transcriptase-Polymerase Chain ReactionKatja Haack, Krishna Soondrum, and Michael NeumaierDifferential DisplayRuey Ho Kao and Giulio FranciaPart III. Mathematical Modeling of MetastasesPredictive Mathematical Modeling in MetastasisJonathan A. Sherratt