Methods For Analysis Of Carbohydrate Metabolism In Photosynthetic Organisms: Plants, Green Algae…

Paperback | September 1, 2016

byHoracio G Pontis

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Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms. The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available. Includes a variety of analytical methods and how to apply the methods using examples from specific case studies Discusses technical information on how to characterize plant carbohydrates and sugar nucleosides Contains easy-to-follow protocols with detailed explanations for self-guidance Provides foundational content as well as step-by-step guidance on how to design and conduct an experiment

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Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include informatio...

Horacio Guillermo Pontis was born in Mendoza, Argentina in 1928. He graduated from the University of Buenos Aires chemistry school and obtained his doctorate working in organic chemistry in 1951. He worked alongside Professor Luis F. Leloir for three years, who influenced his interest in carbohydrate metabolism, sugar phosphates and su...
Format:PaperbackDimensions:250 pages, 8.75 × 6.35 × 0.68 inPublished:September 1, 2016Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0128033967

ISBN - 13:9780128033968

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Table of Contents

Part I. General Analytical Methods Chapter 1.Determination of carbohydrates metabolism molecules

Part II. Purification and analysis of proteins and carbohydrates Chapter 2.Preparation of protein extracts Chapter 3.Protein and carbohydrate separation and purification Chapter 4.Measurement of enzyme activity Chapter 5.General introduction to Mass Spectrometry and Nuclear Magnetic Resonance

Part III. Case studies Chapter 6.Sucrose Chapter 7.Trehalose Chapter 8.Raffinose Chapter 9.Fructans Chapter 10.Polysaccharides Chapter 11.Starch Chapter 12.Glycogen Chapter 13.Cellulose Chapter 14.Sugar phosphates Chapter 15.Nucleotide sugars