Methods in Biological Oxidative Stress by Kenneth HensleyMethods in Biological Oxidative Stress by Kenneth Hensley

Methods in Biological Oxidative Stress

EditorKenneth Hensley, Robert A. Floyd

Paperback | November 9, 2010

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A collection of readily reproducible experimental methods for detecting free radicals and reactive oxygen species or their byproducts. The techniques range from established routine protocols to advanced methodologies using HPLC, mass spectrometry, and electron paramagnetic resonance. The HPLC techniques are applied to the electrochemical measurement of protein oxidation products, particularly nitrotyrosine and dityrosine, to the electrochemical detection of DNA oxidation products. There are also mass spectrometry methods measuring lipid oxidation products.
Title:Methods in Biological Oxidative StressFormat:PaperbackDimensions:232 pages, 9.02 × 5.98 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372005

ISBN - 13:9781617372001

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Table of Contents

Part I. LipidsMeasurement of Fat-Soluble Vitamins and Antioxidants by HPLC With Electrochemical Array DetectionPaul H. Gamache, Paul A. Ullucci, Joe A. Archangelo, and Ian N. AcworthAnalysis of Aldehydic Markers of Lipid Peroxidation in Biological Tissues by HPLC With Fluorescence DetectionMark A. Lovell and William R. MarkesberyMeasurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical IonizationJoshua P. Fessel and L. Jackson Roberts, IIAnalysis of F2-Isoprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical IonizationL. Jackson Roberts, II and Jason D. MorrowMeasurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical IonizationNathalie Bernoud-Hubac and L. Jackson Roberts, IIImmunoassays for Lipid Peroxidation End Products: One-Hour ELISA for Protein-Bound Acrolein and HNEKimihiko Satoh and Koji UchidaFluorometric and Colorimetric Assessment of Thiobarbituric Acid-Reactive Lipid Aldehydes in Biological MatricesKelly S. Williamson, Kenneth Hensley, and Robert A. FloydHPLC With Electrochemical and Photodiode Array Detection Analysis of Tocopherol Oxidation and Nitration Products in Human PlasmaKelly S. Williamson, Kenneth Hensley, and Robert A. FloydPart II. DNA, Protein, and Amino AcidsElectron Paramagnetic Resonance Spin-Labeling Analysis of Synaptosomal Membrane Protein OxidationD. Allan ButterfieldGas Chromatography-Mass Spectrometric Analysis of Free 3-Chlorotyrosine, 3-Bromotyrosine, Ortho-Tyrosine, and 3-Nitrotyrosine in Biological FluidsJoseph P. Gaut, Jaeman Byun, and Jay W. HeineckeIsotope Dilution Gas Chromatography-Mass Spectrometric Analysis of Tyrosine Oxidation Products in Proteins and TissuesJay W. HeineckeSingle-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit AnalysisMark M. HuyckeDetection of Aldehydic DNA Lesions Using Aldehyde Reactive ProbeJun Nakamura and James A. SwenbergAnalysis of Neuroketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass SpectrometryNathalie Bernoud-Hubac, Sean S. Davies, Olivier Boutaud, and L. Jackson Roberts, IIMeasurement of Isoketal Protein Adducts by Liquid Chromatography-Electrospray Ionization/Tandem Mass SpectrometrySean S. Davies, Cynthia J. Brame, Olivier Boutaud, Nathalie Bernoud-Hubac, and L. Jackson Roberts, IIBioassay of 29-Deoxyguanosine/8-Hydroxy-29- Deoxyguanosine by HPLC With Electrochemical/Photodiode Array DetectionKelly S. Williamson, Kenneth Hensley, Quentin N. Pye, Scott Ferrell, and Robert A. FloydHPLC With Electrochemical Detection Analysis of 3-Nitrotyrosine in Human PlasmaKelly S. Williamson, Kenneth Hensley, and Robert A. FloydPart III. Reactive Oxygen Species and Reactive Nitrogen SpeciesProtein Carbonyl Levels-An Assessment of Protein OxidationAlessandra Castegna, Jennifer Drake, Chava Pocernich, and D. Allan ButterfieldFluorogenic Analysis of H2O2 in Biological MaterialsKenneth Hensley, Kelly S. Williamson, and Robert A. FloydDetection of Reactive Oxygen Species by Flow CytometryAlexander Christov, Ladan Hamdheydari, and Paula GrammasNitrite Determination by Colorimetric and Fluorometric Greiss Diazotization Assays: Simple, Reliable, High-Throughput Indices of Reactive Nitrogen Species in Cell Culture SystemsKenneth Hensley, Shenyun Mou, and Quentin N. PyeProtein Carbonyl Determination Using Biotin HydrazideKenneth Hensley and Kelly S. WilliamsonReal-Time, In Vivo Measurement of Nitric Oxide Using Electron Paramagnetic Resonance Spectroscopic Analysis of Biliary FlowKenneth Hensley, Yashige Kotake, Danny R. Moore, Hong Sang, and Lester A. ReinkeIndex