Methods Of Research In Microscopical Anatomy And Embryology

Paperback | October 12, 2012

byCharles Otis Whitman

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1885 edition. Excerpt: ...can now be placed in successive alcohols, stained, and returned to absolute alcohol. It is now to be cleared with creosote or oil of clove, and mounted in the ordinary way. SCHALLIBAUM'S COLLODION FIXATIVE.i The solution, which is prepared by dissolving one part collodion in three or four parts clove oil, is applied to the slide by means of a fine brush, at the time of using. The sections having been arranged, the slide is warmed for a few minutes (5-10) in the oven of a water-bath, in order to evaporate the clove oil. The sections may next be freed from the imbedding mass, and colored according to desire. If the film of collodion be too thick, cloudiness is likely to arise between the sections. The cloudiness can be removed by the use of a brush wet with clove oil, after the sections have been dehydrated by absolute alcohol. Gage,2 who had begun to experiment with collodion before Schallibaum's method was published, recommends that the collodion and clove oil be applied separately:--" A solution of collodion is prepared by adding to 2 grams of gun cotton (that used by photographers is good) 54CC of sulphuric ether and 18T of 95 % alcohol. After the gun cotton is entirely dissolved, the solution should be filtered through filter paper or absorbent cotton. The slides are coated by pouring the collodion on one end, allowing it to flow quickly over the slide and off the other end into the bottle. The prepared slides should be kept free from dust. As the collodion will not deteriorate after drying on the slide, any number of slides may be prepared at the same time. Before using a slide, it should be dusted with a camel's-hair brush; and with another brush the collodionized surface of the slide should be thinly painted with clove oil. 1...

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This historic book may have numerous typos and missing text. Purchasers can usually download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1885 edition. Excerpt: ...can now be placed in successive alcohols, stained, and returned to absolute alcohol. It is now to be cleared wi...

Format:PaperbackDimensions:76 pages, 9.69 × 7.44 × 0.16 inPublished:October 12, 2012Publisher:General Books LLCLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0217514375

ISBN - 13:9780217514378

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