Methods Of Research In Microscopical Anatomy And Embryology

Paperback | May 14, 2012

byCharles Otis Whitman

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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1885 Excerpt: ... Slide allowed to stand several hours (or over night) until the alcohol has completely evaporated, and the sections are left adhering quite firmly to the glass. The process may be hastened by gently warming to 45-50 C. 4. The paraffine may be removed by any of the solvents in common use, but Dr. Gaule recommends xylol. A few drops are allowed to flow over the sections, and after a few moments the paraffine is fully dissolved. 5. The balsam (a mixture of balsam and xylol in equal parts) is placed on the cover-glass, and this allowed to sink slowly, from one side, over the sections. Dr. Gaule finds it convenient, especially with serial sections, to use large cover-glasses, often nearly as large as the slide itself. Thus a single slide may often contain a large number of sections closely arranged under one cover. For large sections, this method offers one important advantage over that of Dr. Giesbrecht; for by the former all wrinkles may be removed, while by the latter the sections must lie as they fall. In the case of smaller sections, not liable to get wrinkled during the placing, I prefer the shellac method. FRENZEL'S GUTTA-PERCHA FIXATIVE.' The only advantage claimed for this fixative is that it allows the sections to be colored after they are fixed on the slide. The difficulty in dissolving the paraffine from the sections is the chief objection to Frenzel's method, which is as follows:--A solution of gutta percha in chloroform and benzine is used. 1 Arch. f. Anat. u. Phys., 1881, Phys. Abthl., p. 156.! Zoologischer Anzeiger, No. 130, p. 51, 1S33. The solution must be filtered until it is clear and almost colorless, and must not be made so thin that it will flow quickly on the slide. The fluid is put on with a brush, and allowed to dry before the sections ...

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This historic book may have numerous typos and missing text. Purchasers can download a free scanned copy of the original book (without typos) from the publisher. Not indexed. Not illustrated. 1885 Excerpt: ... Slide allowed to stand several hours (or over night) until the alcohol has completely evaporated, and the sections are left adh...

Format:PaperbackDimensions:78 pages, 9.69 × 7.44 × 0.16 inPublished:May 14, 2012Publisher:General Books LLCLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0217514405

ISBN - 13:9780217514408

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