MHC Volume 1: A Practical Approach

Paperback | August 1, 1997

EditorN. Fernandez, G. Butcher

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The major histocompatibility complex is the subject of much research in the immunology area. There is great interest in the structure of MHC proteins and in their function as antigen presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetictechniques in order to study these molecules, and these two Practical Approach volumes provide the ideal introduction. MHC 1, Antigen Processing and Presentation, is complemented by MHC 2, Genes, Cells, and Molecules.

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The major histocompatibility complex is the subject of much research in the immunology area. There is great interest in the structure of MHC proteins and in their function as antigen presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetictechniques in order to study these molecules...

Dr Nelson Fernandez, Dept of Biological and Chemical Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK. Tel: 01206 873330. Fax: 01206 872592. Email: nelson@essex.ac.uk Dr Geoff Butcher, Babraham Institute, Babraham, Cambridge, UK

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Format:PaperbackDimensions:236 pages, 9.21 × 6.14 × 0.55 inPublished:August 1, 1997Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635536

ISBN - 13:9780199635535

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Table of Contents

1. Isolation and functional properties of antigen presenting cells2. Labelling methods for analysis of Class II MHC endocytosis and peptide turnover3. Methods for detecting signalling via MHC class II molecules which can lead to either activation or programmed cell death4. Peptide translocation into the ER5. The 20S proteasome and antigen presentation6. Analysis of MHC class II specific T cell clones7. Methods to study the role of the MHC in NK toxicity8. The interaction of superantigens with MHC class II molecules9. Methods for the generation of T cell clones and epithelial cell lines from excised human biopsies or needle aspirates10. The application of mass spectrometry to the analysis of peptides bound to MHC11. The molecular basis of alloreactivity12. Elution and analysis of peptide pools from MHC class I molecules