MHC Volume 2: A Practical Approach by Nelson Fernandez

MHC Volume 2: A Practical Approach

EditorNelson Fernandez, G. Butcher

Paperback | January 1, 1998

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The major histocompatibility complex is the subject of much research in the immunology area. There is great interest in the structure of MHC proteins and in their function as antigen-presenting molecules. Many immunology labs are now faced with the prospect of tackling biochemical and genetictechniques in order to study these molecules, and these two Practical Approach volumes provide the ideal introduction. MHC 2 is complemented by MHC 1.

About The Author

Dr Nelson Fernandez, Dept of Biological and Chemical Sciences, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK. Tel: 01206 873330. Fax: 01206 872592. Email: nelson@essex.ac.uk Dr Geoff Butcher, Babraham Institute, Babraham, Cambridge, UK
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Title:MHC Volume 2: A Practical ApproachFormat:PaperbackDimensions:254 pages, 9.21 × 6.14 × 0.59 inPublished:January 1, 1998Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635552

ISBN - 13:9780199635559

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Table of Contents

1. Generation of HLA-specific human monoclonal antibodies2. Sequencing of proteins isolated by one or two dimensional gel electrophoresis3. Assembly of MHC molecules: requirements of glycosylation, oxidized glutathione, and peptides4. Expression, purification, and characterization of recombinant soluble MHC class I molecules5. The use of pulsed field gel electrophoresis to map the human MHC6. Derivation and analysis of MHC transgenic mice7. Imaging of individual cell surface MHC antigens using fluorescent particles8. Sequence analysis of major histocompatibility molecules9. Approaches to studying MHC expression in mammals other than primates10. Techniques to identify the rules governing class II MHC-peptide formation