Microbes as Tools for Cell Biology

Other | February 1, 1995

byWilson, Leslie, Leslie Wilson

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Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents.

Key Features
* Introduction by renowned microbiologist Dr. Stanley Falkow
* Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes
* Guides researchers in the study of intracellular pathogenesis
* Describes microbial adherence and phagocytosis assays
* Focuses on protein trafficking in infected cells
* Well-illustrated with color plates, halftones, and diagrams

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From the Publisher

Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of ...

Format:OtherDimensions:339 pages, 1 × 1 × 1 inPublished:February 1, 1995Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080859437

ISBN - 13:9780080859439

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Table of Contents

D.G. Russell, Preface.
S. Falkow, Introduction.
D.G. Russell, Obtaining and Maintaining Microbial Pathogens.
Manipulation of Pathogens:
W. Trager, Cultivation of Malaria Parasites.
D.S. Roos, R.G.K. Donald, N.S. Morrissette, and A.L.C. Moulton, Molecular Tools for Genetic Dissection of the Protozoan ParasiteToxoplasma gondii.
J.H. LeBowitz, Transfection Experiments withLeishmania.
R. Tsolis and F. Heffron, Mutagenesis and Variant Selection inSalmonella.
N.D. Connell, Mycobacterium: Isolation, Maintenance, Transformation, and Mutant Selection.
Microbial Adherence and Invasion Assays:
G.J. Bancroft, H.L. Collins, L.B. Sigola, and C.E. Cross, Modulation of Murine Macrophage Behaviorin Vivoandin Vitro.
E.J. Brown, In VitroAssays of Phagocytic Function of Human Peripheral Blood Leukocytes: Receptor Modulation and Signal Transduction.
P. Falk, T. Borén, D. Haslam, and M. Caparon, Bacterial Adhesion and Colonization Assays.
I. Crandall and I.W. Sherman, Cytoadherence and thePlasmodium falciparum-Infected Erythrocyte.
The Study of Intracellular Pathogenesis:
M.J. Blackman, Purification ofPlasmodium falciparumMerozoites for Analysis of the Processing of Merozoite Surface Protein-1.
K. Haldar, H.G. Elmendorf, A. Das, W.L. Li, D.J.P. Ferguson, and B.C. Elford, In VitroSecretory Assays with Erythrocyte-Free Malaria Parasites.
K.H. Berger and R.R. Isberg, Intracellular Survival byLegionella.
P. Chakraborty, S. Sturgill-Koszycki, and D.G. Russell, Isolation and Characterization of Pathogen-Containing Phagosomes.
D.G. Russell, Immunoelectron Microscopy of Endosomal Trafficking in Macrophages Infected with Microbial Pathogens.
P.H. Schlesinger, Measuring the pH of Pathogen-Containing Phagosomes.
C.V. Harding, Techniques for Studying Phagocytic Processing of Bacteria for Class I or II MHC-Restricted Antigen Recognition by T Lymphocytes.
Chapter References.
Subject Index.
Volumes in Series.