Mitochondria

Other | May 1, 2001

byWilson, Leslie, Leslie Wilson

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This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs.
The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls. There is a unique set of appendices that include gene catalogs, mtDNA maps, and reagents for probing respiratory chain function. Finally, there are applications of state-of-the art microarray and gene chip technologies.

Key Features
* Isolation of mitochondria from commonly used cells and tissues
* Assays for mitochondrial activities, including respiration, ATP production, permeability, protein import, and interactions with the cytoskeleton
* Biochemical and optical methods for studying protein-protein interactions in mitochondria
* Approaches to studying mitochondrial replication, transcription, and translation
* Transmitochondrial technologies
* Methods in microassay data analysis

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From the Publisher

This title employs biochemical, cell biological, and genetic approaches to study mitochondrial structure, function, and biogenesis. Also of interest are the consequences of impaired mitochondrial function on cells, tissues, and organs. The book is full of step-by-step "how to" methods with sample results, interpretations, and pitfalls....

Format:OtherDimensions:519 pages, 1 × 1 × 1 inPublished:May 1, 2001Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:008052253X

ISBN - 13:9780080522531

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Table of Contents

Contributors.
Preface.
Isolation and Subfractionation of Mitocondria from Animal Cells and Tissue Culture Lines,F. Pallotti and G. Lenaz.
Isolation and Subfractionation of Mitochondria from the YeastSaccharomyces cerevisiae,K. Dierkert, A.I.P.M. de Kroon, G. Kispal, and R. Lill.
Isolation and Subfractionation of Mitochondria from Plants,A.H. Miller, A. Liddell, and C.J. Leaver.
Assessing Functional Integrity of Mitochondriain Vitroandin Vivo,M.D. Esposti.
Assaying Mitochondrial Respiratory Complex Activity in Mitochondria Isolated from Human Cells and Tissues,M.A. Birch-Machin and D.M. Turnbull.
In VivoMeasurements of Respiration Control by CytochromecOxidase andin SituAnalysis of Oxidative Phosphorylation,G. Villani and G. Attardi.
Assay of Mitochondrial ATP Synthesis in Animal Cells,G. Manfredi, A. Spinazzola, N. Checcarelli, and A. Naini.
Measurement of Membrane Permeability and Permeability Transition of Mitochondria,N. Zamzami, C. Maisse, D. Métivier, and G. Kroemer.
Assaying Actin-Binding Activity of Mitochondria in Yeast,I.R. Boldogh and L.A. Pon.
Analysis and Prediction of Mitochondrial Targeting Peptides,O. Emanuelsson, G. von Heijne, and G. Schneider.
Assaying Protein Import into Mitochondria,M.T. Ryan, W. Voos, and N. Pfanner.
Analysis of Protein-Protein Interactions in Mitochondria by Coimmunoprecipitation and Chemical Cross-Linking,J.M. Herrmann, B. Westermann, and W. Neupert.
Blue-Native Gels to Isolate Protein Complexes from Mitochondria,H. Schägger.
Application of Electron Tomography to Mitochondrial Research,C.A. Mannella.
Epitope Tagging and Visualization of Nuclear-Encoded Mitochondrial Proteins in Yeast,D.W. Nowakowski, T.C. Swayne, and L.A. Pon.
Targeting of Green Fluorescent Protein to Mitochondria,K. Okamoto, P.S. Perlman, and R.A. Butow.
Assessment of Mitochondrial Membrane Potentialin SituUsing Single Potentiometric Dyes and Novel Fluorescence Resonance Energy,J.A. Dykens and A.K. Stout.
Optical Imaging Techniques (Histochemical, Immunohistochemical, andin SituHybridization Staining Methods) to Visualize Mitochondria,K. Tanji and E. Bonilla.
Visualization of Mitochondrial Movement in Yeast,H.-C. Yang, V. Simon, T. Swayne, and L. Pon.
Targeting of Reporter Molecules to Mitochondria to Measure Calcium, ATP, and pH,A.M. Porcelli, P. Pinton, E.K. Ainscow, A. Chiesa, M. Rugolo, G.A. Rutter, and R. Rizzuto.
Genetic Transformation ofSaccharomyces cerevisiaeMitochondria,N. Bonnefoy and T.D. Fox.
Transmitochondrial Technology in Animal Cells,C.T. Moraes, R. Dey, and A. Barrientos.
Diagnostic Assays for Defects in Mitochondrial DNA Replication and Transcription in Yeast and Human Cells,B.L. Seidel-Rogol and G.S. Shadel.
Analysis of Mitochondrial Translation Productsin Vivoandin Organelloin Yeast,B. Westermann, J.M. Herrmann, and W. Neupert.
Numerical Methods for Handling Uncertainty in Microarray Data: An Example Analyzing Perturbed Mitochondrial Function in Yeast,C.B. Epstein, W. Hale, IV, and R.A. Butow.
Appendices.
Index.
Volumes in Series.